Handley C J, Lowther D A
Biochim Biophys Acta. 1976 Aug 24;444(1):69-74. doi: 10.1016/0304-4165(76)90224-5.
The depression of proteoglycan synthesis in ten-day-old high density chondrocyte cultures was shown to be dependent on both the concentration and time of exposure of the cells to hyaluronic acid. Hyaluronic acid had no effect on the overall protein synthesis by the cultured cells. Using benzyl-beta-D-xyloside an exogenous acceptor, it was shown that glycosaminoglycan biosynthesis by the chondrocytes was not affected by hyaluronic acid. It was concluded that hyaluronic acid was effecting glycosaminoglycan chain initiation, hence proteoglycan biosynthesis, either by specifically depressing the synthesis of the core protein or by repressing the activity of the xylosyltransferase.
研究表明，在十天龄的高密度软骨细胞培养物中，蛋白聚糖合成的抑制取决于细胞暴露于透明质酸的浓度和时间。透明质酸对培养细胞的整体蛋白质合成没有影响。使用苄基-β-D-木糖苷作为外源性受体，结果表明软骨细胞的糖胺聚糖生物合成不受透明质酸影响。得出的结论是，透明质酸通过特异性抑制核心蛋白的合成或抑制木糖基转移酶的活性来影响糖胺聚糖链的起始，从而影响蛋白聚糖的生物合成。
