Handley C J, Lowther D A
Biochim Biophys Acta. 1977 Nov 7;500(1):132-9. doi: 10.1016/0304-4165(77)90053-8.
Proteoglycan biosynthesis by cultured chondrocytes was shown to be depressed by extracellular concentrations of proteoglycan and partially degraded proteoglycan. This reduction in proteoglycan synthesis was reversible on removal of the added proteoglycan. Benzyl-beta-D-xyloside, an exogenous acceptor of glycosaminoglycan synthesis, was used and it was shown that proteoglycan was inhibiting glycosaminoglycan synthesis. Proteoglycan had no effect on the overall protein synthesis by the cultured cells. It was concluded that the exogenous proteoglycan was inhibiting proteoglycan synthesis at the level of initiation or elongation of the glycosaminoglycan chains.
培养的软骨细胞进行蛋白聚糖生物合成时，细胞外浓度的蛋白聚糖和部分降解的蛋白聚糖会抑制其合成。去除添加的蛋白聚糖后，蛋白聚糖合成的这种减少是可逆的。使用了糖胺聚糖合成的外源性受体苄基-β-D-木糖苷，结果表明蛋白聚糖会抑制糖胺聚糖的合成。蛋白聚糖对培养细胞的整体蛋白质合成没有影响。得出的结论是，外源性蛋白聚糖在糖胺聚糖链起始或延长水平上抑制蛋白聚糖合成。
