[大鼠肝细胞对3H-皮质酮的受体结合]
[The receptor binding of 3H-corticosterone by rat hepatocytes].
作者信息
Panin L E, Trubitsyna O M, Tret'iakova T A, Usynin I F
出版信息
Biull Eksp Biol Med. 1992 Oct;114(10):362-4.
The binding of 3H-corticosterone was studied on rat hepatocytes both in presence of unlabeled corticosterone, obsidan and their absence at 0 degrees-4 degrees C. The analysis of binding by the method of Scatchard showed that there are two types of specific binding sites for 3H-corticosterone. Possible existence of proper glucocorticoid receptors (Ka = 4 x 10(9)M-1, n = 0.52 x 10(-14) mol/mg prot.) has been shown, as well as possibility of 3H-corticosterone interaction with beta-adrenoreceptors (Ka = 1.2 x 10(9)M-1, n = 0.9 x 10(-14) mol/mg prot.) have been demonstrated on hepatocytes.
在0℃至4℃条件下，分别在有无未标记的皮质酮、黑曜石存在的情况下，研究了3H-皮质酮与大鼠肝细胞的结合情况。用Scatchard法分析结合情况表明，3H-皮质酮存在两种特异性结合位点。已证明可能存在合适的糖皮质激素受体（Ka = 4×10⁹M⁻¹，n = 0.52×10⁻¹⁴mol/mg蛋白），同时也证明了3H-皮质酮与肝细胞上的β-肾上腺素能受体相互作用的可能性（Ka = 1.2×10⁹M⁻¹，n = 0.9×10⁻¹⁴mol/mg蛋白）。
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