Horisberger M A
Ciba-Geigy Ltd., Biotechnology, Basle, Switzerland.
J Interferon Res. 1992 Dec;12(6):439-44. doi: 10.1089/jir.1992.12.439.
Interferon-alpha (IFN-alpha) and -gamma differed in their action against influenza virus and vesicular stomatitis virus (VSV) on pig cells. Recombinant IFN-alpha severely impaired the cytopathic effect of VSV on PK-15 cells, whereas recombinant porcine IFN-gamma did not. IFN-alpha impaired also the replication of VSV and of influenza virus in primary pig kidney cells in contrast to IFN-gamma, which failed to induce an efficient antiviral state against both viruses. Otherwise, the IFN system seemed to work properly in pig cells since both IFN-alpha and IFN-gamma induced an efficient antiviral state to mengovirus. The establishment of the antiviral state to VSV and influenza virus correlated with the induction of two cytoplasmic proteins related to the murine Mx protein involved in the selective resistance of mice to influenza virus infection. The results are discussed in the context of the susceptibility of pigs to influenza virus strains that are in circulation in birds and in humans.
α干扰素（IFN-α）和γ干扰素对猪细胞上的流感病毒和水疱性口炎病毒（VSV）的作用有所不同。重组IFN-α严重损害了VSV对PK-15细胞的细胞病变效应，而重组猪IFN-γ则没有。与IFN-γ相反，IFN-α也损害了VSV和流感病毒在原代猪肾细胞中的复制，IFN-γ未能诱导针对这两种病毒的有效抗病毒状态。否则，IFN系统在猪细胞中似乎正常发挥作用，因为IFN-α和IFN-γ都能诱导针对脑心肌炎病毒的有效抗病毒状态。对VSV和流感病毒的抗病毒状态的建立与两种细胞质蛋白的诱导相关，这两种蛋白与参与小鼠对流感病毒感染的选择性抗性的小鼠Mx蛋白有关。本文在猪对在鸟类和人类中传播的流感病毒株的易感性背景下讨论了这些结果。
