链霉菌ATP核苷酸3'-焦磷酸激酶及其基因。

Streptomyces ATP nucleotide 3'-pyrophosphokinase and its gene.

作者信息

Muta S, Osoegawa K, Ezaki S, Zubair M, Kuhara S, Mukai J, Dixon R

机构信息

Laboratory of Molecular Gene Technics, Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.

出版信息

Nucleic Acids Symp Ser. 1992(27):165-6.

PMID:1337783

Abstract

Streptomyces ATP nucleotide 3'-pyrophosphokinase is an extracellular, ribosome-independent, and stringent factor-mimic ppGpp synthetase with an unusually broad acceptor spectrum. The gene-containing DNA fragments cloned from chromosomal DNA of a producer S. morookaensis into pIJ699 and pUC plasmids were found to express the active enzyme in the transformed S. lividans TK24 and enteric E. coli JM109 and nitrogen-fixing Klebsiella pneumoniae M5a1 and 5022, respectively. Base sequence of the structural gene and the deduced amino acid sequence exhibited little homology to those of E. coli stringent factor and related proteins. Growth retardation was seen in some transformants.

摘要

链霉菌ATP核苷酸3'-焦磷酸激酶是一种细胞外、不依赖核糖体且模拟严谨因子的ppGpp合成酶，具有异常广泛的受体谱。从产链霉菌莫罗卡链霉菌的染色体DNA克隆到pIJ699和pUC质粒中的含基因DNA片段，分别在转化的变铅青链霉菌TK24、肠道大肠杆菌JM109和固氮肺炎克雷伯菌M5a1及5022中表达出活性酶。结构基因的碱基序列和推导的氨基酸序列与大肠杆菌严谨因子及相关蛋白的序列几乎没有同源性。在一些转化体中观察到生长迟缓。