Reguli J, Duraćková Z, Pogády J, Martisová D, Stasko A
Katedra fyzikálnej chémie CHTF STU v Bratislave, CSFR.
Bratisl Lek Listy. 1992 Nov;93(11):557-67.
Respiratory burst during phagocytosis of human neutrophils is connected with the production of superoxide anion radical O2.-. This radical changes into further bioreactive oxygen products (especially H2O2 and .OH) which can be harmful to the organism. The principles of EPR spin trapping, the most reliable method for the study of short living free radicals, are presented. Advantages and problems of the study of O2.- and .OH by means of the 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) spin trap were assessed. Overproduction of O2.- (e.g. by chronic inflammation) can be treated with superoxide dismutase (SOD) or other drugs with SOD-like activity. Methods for studying such superoxide scavengers are reviewed. (Fig. 3, Tab. 2, Ref. 88.).
人类中性粒细胞吞噬过程中的呼吸爆发与超氧阴离子自由基O2.-的产生有关。该自由基会进一步转变为具有生物活性的氧产物（尤其是H2O2和.OH），这些产物可能对机体有害。本文介绍了电子顺磁共振自旋捕集技术的原理，这是研究短寿命自由基最可靠的方法。评估了利用5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）自旋捕集技术研究O2.-和.OH的优缺点。O2.-的过量产生（如慢性炎症所致）可用超氧化物歧化酶（SOD）或其他具有SOD样活性的药物进行治疗。本文综述了研究此类超氧化物清除剂的方法。（图3，表2，参考文献88篇）
