从刺槐种子凝集素中分离得到的糖肽碳水化合物链的结构分析。
Structural analysis of the carbohydrate chain of glycopeptides isolated from Robinia pseudoacacia seed lectins.
作者信息
Wantyghem J, Platzer N, Giner M, Derappe C, Goussault Y
机构信息
Unité INSERM 180-UAC CNRS 71, Paris, France.
出版信息
Carbohydr Res. 1992 Dec 15;236:181-93. doi: 10.1016/0008-6215(92)85015-r.
Robinia pseudoacacia seeds contain lectins which are closely related. Pronase digestion of the dimeric and tetrameric lectins, RPA1 and RPA3, gave glycopeptides. The structure of the oligosaccharide was determined by 1H NMR spectroscopy and carbohydrate determination as alpha-D-Manp-(1-->3)-[beta-D-Xylp-(1-->2)]-[alpha-D-Manp+ ++-(1-->6)]-beta- D-Manp-(1-->4)-beta-D-GlcpNAc-(1-->4)-[alpha-L-Fucp-(1-->3)] -beta-D-GlcpNAc - (1-->4)-Asn. It appears that the 34-kDa constitutive polypeptide of RPA1 contains 4-5 carbohydrate chains whereas the 30.5-kDa and 29-kDa subunits of RPA3 contain two and one oligosaccharide chains, respectively.
刺槐种子含有密切相关的凝集素。用链霉蛋白酶消化二聚体和四聚体凝集素RPA1和RPA3，得到糖肽。通过1H核磁共振光谱和碳水化合物测定确定了寡糖的结构为α-D-甘露糖基-(1→3)-[β-D-木糖基-(1→2)]-[α-D-甘露糖基+++(1→6)]-β-D-甘露糖基-(1→4)-β-D-葡萄糖胺基-(1→4)-[α-L-岩藻糖基-(1→3)]-β-D-葡萄糖胺基-(1→4)-天冬酰胺。似乎RPA1的34 kDa组成型多肽含有4-5条碳水化合物链，而RPA3的30.5 kDa和29 kDa亚基分别含有两条和一条寡糖链。
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