Halmy L, Fehér T
Endokrinologie. 1976 Jun;67(2):184-91.
[4-14C]-Dehydroepiandrosterone and [7 alpha-3H]-Dehydroepiandrosterone sulphate were injected simultaneously to normal and obese female subjects. The percentage recovery of 14C and 3H radioactivies in dehydroepiandrosterone sulphate, androsterone sulphate, etiocholanolone sulphate, androsterone glucuronoside and etiocholanolone glucuronoside was determined in the day-to-day urine collections for 72 hr. Results showed a normal total 3H recovery and a poor 14C recovery in urinary conjugates of obese patients. The rate of appearance of 3H activity was not identical in the individual metabolites of normal subjects, and it was not normal in obesity. Overweight subjects exhibited an acceleration in [7 alpha-3H]-Dehydroepiandrosterone sulphate metabolism to androsterone glucuronoside. The observation regarding the rate of appearance of urinary conjugates bearing 14C isotope correlate with our previous finding in which a glandular overproduction of free dehydroepiandrosterone was found and an uptake of this steroid by the adipose tissue was suggested. Our results showed that the poor recovery of 14C radioactivity in urine of obese female subjects was not an aspecific consequence of illness.
将[4-¹⁴C]-脱氢表雄酮和[7α-³H]-硫酸脱氢表雄酮同时注射到正常和肥胖女性受试者体内。在连续72小时的日常尿液收集样本中，测定硫酸脱氢表雄酮、硫酸雄酮、硫酸本胆烷醇酮、雄酮葡萄糖醛酸苷和本胆烷醇酮葡萄糖醛酸苷中¹⁴C和³H放射性的回收率。结果显示，肥胖患者尿液结合物中³H的总回收率正常，而¹⁴C的回收率较差。正常受试者个体代谢物中³H活性的出现速率并不相同，在肥胖个体中也不正常。超重受试者表现出[7α-³H]-硫酸脱氢表雄酮代谢为雄酮葡萄糖醛酸苷的加速。关于带有¹⁴C同位素的尿液结合物出现速率的观察结果与我们之前的发现相关，即发现游离脱氢表雄酮腺体过量产生，并提示脂肪组织对这种类固醇有摄取。我们的结果表明，肥胖女性受试者尿液中¹⁴C放射性回收率低并非疾病的非特异性后果。
