Kleinhans F W, Travis V S, Du J, Villines P M, Colvin K E, Critser J K
Center for Reproduction and Transplantation Immunology, Methodist Hospital, Indianapolis, Indiana 46202.
J Androl. 1992 Nov-Dec;13(6):498-506.
An electron spin resonance technique using the spin label tempone and the broadening agent potassium chromium oxalate was used to measure the water volume of human sperm. The toxicity of tempone (5 mmol/L) and potassium chromium oxalate (50 mmol/L) to sperm was measured over a time span of 120 minutes using computer-assisted semen analysis. Tempone had no effect on any computer-assisted semen analysis parameters, including motility. Potassium chromium oxalate reduced sperm motility by an average of 24% during the first 30 minutes of exposure. After selection by swim-up and correction for the presence of dead cells and cytoplasmic droplets, a water volume of 20.0 +/- 2.9 microns3 was obtained. This yields a total volume of 33.9 microns3 if a water compartment of 59% by volume is assumed. These results are consistent with other shape-independent techniques for measuring volume, but larger than the generally accepted optical and electronic particle counter sizes.
采用自旋标记物TEMPONE和增宽剂草酸铬钾的电子自旋共振技术来测量人类精子的水体积。使用计算机辅助精液分析，在120分钟的时间跨度内测量了TEMPONE（5毫摩尔/升）和草酸铬钾（50毫摩尔/升）对精子的毒性。TEMPONE对任何计算机辅助精液分析参数均无影响，包括活力。草酸铬钾在暴露的前30分钟内使精子活力平均降低了24%。经过上浮法筛选并校正死细胞和细胞质滴的存在后，得到的水体积为20.0±2.9立方微米。如果假设水相体积占59%，则总体积为33.9立方微米。这些结果与其他不依赖形状的体积测量技术一致，但大于普遍接受的光学和电子粒子计数器测量的尺寸。
