Fetic Sabina, Yeung Ching-Hei, Sonntag Barbara, Nieschlag Eberhard, Cooper Trevor G
Institute of Reproductive Medicine, University of Münster, Germany.
J Androl. 2006 Mar-Apr;27(2):294-301. doi: 10.2164/jandrol.05122.
A significantly greater percentage of motile than immotile spermatozoa bore droplets at the osmolality of semen and cervical mucus. The percentage of spermatozoa with droplets was not significantly correlated with the osmolality of semen or the extent of cell swelling in response to quinine in hypotonic medium. Droplets appeared slightly more frequently (ie, were more obvious) in the presence of quinine, which blocks regulatory volume decrease, indicating that they are the major site of volume expansion. There was no selection for or against droplet-bearing spermatozoa migrating through viscous surrogate mucus at high or low osmolality. Sperm swelling in response to quinine at mucus osmolality was significantly greater in fathers than in patients whose partners had no fertility problem. Therefore, cytoplasmic droplets are not deleterious to sperm motility and may be related to physiological volume regulation, which may be predictive of some forms of human male infertility.
在精液和宫颈黏液的渗透压条件下，活动精子携带液滴的比例显著高于不活动精子。携带液滴的精子百分比与精液渗透压或低渗介质中奎宁刺激引起的细胞肿胀程度无显著相关性。在存在奎宁（可阻断调节性容积减小）的情况下，液滴出现的频率略高（即更明显），这表明它们是容积扩张的主要部位。在高渗或低渗条件下，携带或不携带液滴的精子在通过黏性替代黏液迁移时不存在选择偏好。在黏液渗透压条件下，父亲精子对奎宁刺激的肿胀反应显著大于其配偶无生育问题的患者精子。因此，细胞质液滴对精子活力无害，可能与生理容积调节有关，这可能对某些形式的男性不育具有预测作用。
