Kosowska B
Department of Genetics and Animal Husbandry, Agricultural University, Wrocław, Poland.
Biochem Genet. 1992 Dec;30(11-12):581-9. doi: 10.1007/BF02399808.
Male and female wild Norway rats (Rattus norvegicus Erxleben) and males and female albino outbred rats (Ipf:RIZ) were crossbred. The resulting animals (F1 hybrids) were the control, noninbred group (0% inbred). By systematic full-sib mating, two experimental groups (50 and 91% of inbred) were produced. Half of each group (both males and females) was exposed to physical stress (3 days of starvation and 3 hr of swimming). The other half of each group was anesthetized using ether to collect blood. The anterior pituitary hormone concentrations of prolactin (PRL), corticotropin (ACTH), and growth hormone (rGH) in blood serum were determined by the radioimmunoassay method. Significant relationships between the PRL, ACTH, and rGH concentrations in blood serum and the inbreeding coefficient were observed: A significant PRL content decrease in blood serum occurred (linear function) and the rGH and ACTH content diminished significantly rapidly (quadratic function). These changes were affected by an increase in homozygosity. Stress significantly influenced PRL, ACTH, and rGH concentrations as well. The sex of rats significantly determined PRL and ACTH content only. Hormone levels were also influenced by interactions between the factors studied (inbred level, sex, stress).
将雄性和雌性野生挪威大鼠（褐家鼠，Erxleben）以及雄性和雌性白化远交大鼠（Ipf:RIZ）进行杂交。所产生的动物（F1杂种）作为对照，即非近亲繁殖组（近亲繁殖率为0%）。通过系统的全同胞交配，产生了两个实验组（近亲繁殖率分别为50%和91%）。每组中的一半动物（雄性和雌性）接受身体应激（3天饥饿和3小时游泳）。每组的另一半动物用乙醚麻醉以采集血液。采用放射免疫分析法测定血清中催乳素（PRL）、促肾上腺皮质激素（ACTH）和生长激素（rGH）的垂体前叶激素浓度。观察到血清中PRL、ACTH和rGH浓度与近亲繁殖系数之间存在显著关系：血清中PRL含量显著下降（线性函数），rGH和ACTH含量显著快速下降（二次函数）。这些变化受到纯合性增加的影响。应激也显著影响PRL、ACTH和rGH的浓度。大鼠的性别仅显著决定PRL和ACTH的含量。激素水平还受到所研究因素（近亲繁殖水平、性别、应激）之间相互作用的影响。
