Gami-Yilinkou R, Kaliszan R
Department of Biophamaceutics and Pharmacodynamics, Medical Academy, Gdańsk, Poland.
Pol J Pharmacol Pharm. 1992 Sep-Oct;44(5):515-25.
Two new commercially available reversed phase high performance liquid chromatographic (HPLC) columns were tested from the view point of their usefulness for determination of hydrophobicity by means of partition chromatography. One column comprised a specially prepared hydrocarbon-bonded silica stationary phase material. The other column was packed with a polybutadiene-coated alumina (PBCA) phase. As reference served a regular commercial octadecylsilica (ODS) column. A series of test solutes were pyrazine CH- and NH-acids--the compounds which are able to take part in specific as well as in nonspecific intermolecular interactions. Unique properties of the new hydrocarbonaceous column manifested themselves in regular linear relationships between logarithms of capacity factors and volume percent of methanol in aqueous eluent. The main advantage of the PBCA column is a possibility of performing chromatography at alkaline pH. Both new columns are superior to regular ODS columns in respect of providing reliable chromatographic measures of hydrophobicity. It was observed that hydrophobicity parameters determined in individual HPLC systems are not highly intercorrelated and hence can reflect different structural features of solutes.
从通过分配色谱法测定疏水性的实用性角度出发，对两种新上市的反相高效液相色谱（HPLC）柱进行了测试。其中一根色谱柱包含一种特殊制备的碳氢化合物键合硅胶固定相材料。另一根色谱柱填充的是聚丁二烯涂覆的氧化铝（PBCA）相。作为参考的是常规市售的十八烷基硅胶（ODS）柱。一系列测试溶质为吡嗪CH-酸和NH-酸，这些化合物能够参与特异性以及非特异性分子间相互作用。新型含烃色谱柱的独特性能表现为容量因子对数与水性洗脱液中甲醇体积百分比之间呈现规则的线性关系。PBCA色谱柱的主要优点是能够在碱性pH条件下进行色谱分析。就提供可靠的疏水性色谱测定方法而言，这两种新型色谱柱均优于常规ODS柱。据观察，在各个HPLC系统中测定的疏水性参数并非高度相互关联，因此能够反映溶质的不同结构特征。
