Vollinga R C, Zuiderveld O P, Scheerens H, Bast A, Timmerman H
Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
Methods Find Exp Clin Pharmacol. 1992 Dec;14(10):747-51.
A simple and rapid functional test system for the screening of histamine H3 ligands is described. It is based on the inhibitory effect of histamine H3 agonists on electrically-evoked contractile response of isolated guinea pig intestine. Whole jejunum segments are continuously stimulated maximally (15 V) by electrical pulses with a frequency of 0.1 Hz and a duration of 0.5 msec. The resulting twitches are recorded isotonically (1.0 g) and can be completely abolished by atropine (0.1 mcM).
本文描述了一种用于筛选组胺H3配体的简单快速的功能测试系统。该系统基于组胺H3激动剂对离体豚鼠肠电诱发收缩反应的抑制作用。空肠全段以0.1 Hz的频率和0.5毫秒的持续时间通过电脉冲进行最大程度的连续刺激（15 V）。由此产生的抽搐以等张方式（1.0 g）记录，并且可被阿托品（0.1 μM）完全消除。
