Liu Z H
Jinling Hospital, Nanjing.
Zhonghua Yi Xue Za Zhi. 1992 Oct;72(10):593-5, 638-9.
Epidermal growth factor (EGF) and its receptor (EGF-R) were identified by immunohistochemical method (4 layer PAP) in the renal tissue specimens obtained from 11 normal kidneys and 17 cases of acute renal failure (ARF). The quantitative EGF and EGF-R in the tissue were expressed as positive tubules per mm2. The amount of EGF and EGF-R in renal tissue was markedly increased in cases of ARF than in normal persons. Positive EGF staining was present only in distal tubules, but absent from glomeruli in normal persons. In ARF patients EGF was identified in both distal and proximal tubules. It was also seen in the glomerular mesangial area in 2 cases complicated by mesangial proliferative GN. However, no obvious difference was noted in the distribution of EGF-R between normal and ARF specimens. The amount of EGF in the renal tissue varied with the morphological changes in tubules and the time when the renal biopsy was being taken. The amount of EGF was large in those with a Scr value less than 2.5mg/dl There was a more profound expression of EGF during the recovery from ARF.
采用免疫组织化学方法（四层PAP法），对11例正常肾脏及17例急性肾衰竭（ARF）患者的肾组织标本进行表皮生长因子（EGF）及其受体（EGF-R）的检测。组织中EGF和EGF-R的定量以每平方毫米阳性肾小管数表示。ARF患者肾组织中EGF和EGF-R的含量明显高于正常人。正常人中，EGF阳性染色仅见于远端小管，肾小球无阳性染色。ARF患者中，EGF在远端和近端小管均有表达。在2例合并系膜增生性肾小球肾炎（GN）的患者中，肾小球系膜区也可见EGF。然而，正常标本与ARF标本中EGF-R的分布无明显差异。肾组织中EGF的含量随肾小管形态变化及肾活检时间而不同。血清肌酐（Scr）值小于2.5mg/dl的患者，其肾组织中EGF含量较高。ARF恢复期EGF表达更为明显。
