Chan P J, Su B C, Tredway D R, Seraj M, Seraj I M, King A
Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, California 92350.
J Assist Reprod Genet. 1992 Dec;9(6):531-3. doi: 10.1007/BF01204249.
The purpose of this study was to determine if oocytes were capable of taking up exogenous DNA such as human papillomaviral (HPV) DNA and evaluate the zona pellucida as a barrier to the entry of foreign DNA into the oocyte.
The experiment consisted of four groups of hamster oocytes exposed to HPV DNA fragments: Group A, zona-free oocytes (n = 5); Group B, oocytes with an intact zona pellucida (n = 5); Group C, oocytes fixed in 4% buffered formalin solution for 20 min (n = 5); and Group D, zona-free oocytes (n = 4). Group C oocytes served as an internal control to ensure adequate washing of the oocytes after incubation.
The zona pellucida was not a barrier to foreign DNA molecules. The PCR did not detect L1-HPV and beta-globin gene sequences in the untreated hamster oocyte. Uptake of the smaller DNA fragments such as that amplified from the beta-globin region was independent of active oocyte cell processes.
Oocytes cultured in vitro can passively take up exogenous DNA fragments. The results suggest a possible role of oocytes as vectors for foreign DNA.
本研究旨在确定卵母细胞是否能够摄取外源性DNA，如人乳头瘤病毒（HPV）DNA，并评估透明带作为外源DNA进入卵母细胞的屏障作用。
实验包括四组暴露于HPV DNA片段的仓鼠卵母细胞：A组，无透明带卵母细胞（n = 5）；B组，透明带完整的卵母细胞（n = 5）；C组，在4%缓冲福尔马林溶液中固定20分钟的卵母细胞（n = 5）；D组，无透明带卵母细胞（n = 4）。C组卵母细胞作为内部对照，以确保孵育后卵母细胞充分洗涤。
透明带对外源DNA分子不是屏障。PCR未在未处理的仓鼠卵母细胞中检测到L1-HPV和β-珠蛋白基因序列。较小DNA片段（如从β-珠蛋白区域扩增的片段）的摄取与卵母细胞的主动细胞过程无关。
体外培养的卵母细胞可被动摄取外源DNA片段。结果提示卵母细胞可能作为外源DNA的载体。
