Chen J F, Chen W L, Liang P, Zhong X R
Laboratory of Electron Microscopy, Fujian Medical College, Fuzhou.
Chin Med J (Engl). 1992 Dec;105(12):1009-13.
The results of ultracytochemical studies on Trichomonas hominis showed that ACPase and CMPase were mainly located in the mature face sacs of the primary lysosomes, digestive vacuoles, as well as in the parabasal body. TPPase and NADPase were found in the saccules at the mature face and the intermediate saccules of parabasal body respectively. This study revealed that T. hominis had well-developed parabasal bodies. Negative COase and catalase reactions indicated that T. hominis lacked both mitochondria and microbody. Hydrogenosome was stained well with the Ur-Pb-Cu impregnation technique.
人毛滴虫超微细胞化学研究结果表明,酸性磷酸酶(ACPase)和胞嘧啶核苷酸酶(CMPase)主要位于初级溶酶体、消化泡的成熟面囊以及副基体中。焦磷酸酶(TPPase)和烟酰胺腺嘌呤二核苷酸磷酸酶(NADPase)分别见于副基体成熟面的扁平囊和中间扁平囊。本研究揭示人毛滴虫具有发育良好的副基体。一氧化碳酶(COase)和过氧化氢酶反应阴性表明人毛滴虫既没有线粒体也没有微体。氢化酶体用铀-铅-铜浸染技术染色良好。