Rambourg A, Clermont Y, Hermo L
Département de Biologie du CEA, Saclay, France.
Am J Anat. 1988 Nov;183(3):187-99. doi: 10.1002/aja.1001830302.
The three-dimensional structure of the Golgi apparatus and its components has been analyzed in sections of pancreatic acinar cells by using stereopairs of electron microscope photographs. Pancreatic tissue fixed in glutaraldehyde was postfixed in reduced osmium, and the sections were stained with lead citrate. Tissues were also treated to demonstrate phosphatase activity (i.e., nicotinamide adenine dinucleotide phosphatase, NADPase; thiamine pyrophosphatase, TPPase; cytidine monophosphatase, CMPase). The following stacked components were observed along the branching, anastomotic, continuous, ribbonlike Golgi apparatus. 1) On the cis-face of the Golgi stack there was a tubular membranous network known to be osmiophilic and referred to as the cis-osmiophilic tubular network or cis-element. 2) A first, poorly fenestrated saccule, unreactive for the phosphatases tested, was slightly distended in places and contained a fluffy granulofilamentous material. 3) The subjacent three or four saccules, reactive for NADPase and/or TPPase, showed dilated portions containing a granulofilamentous secretory material similar to that filling the rest of the saccule. They also showed nondilated portions perforated with large fenestrations, some of which were in register and formed wells containing 80-nm vesicles. The dilated portions of these saccules were present at random along the length of the saccules and were not located exclusively at their edges. 4) The remaining one or two elements of the stack, CMPase positive, showed dilated spheroidal portions or prosecretory granules containing a homogeneous secretory material and flattened fenestrated regions free of secretory material and having the appearance of networks of narrow membranous tubules. 5) Lastly on the trans-aspect of the stack there were detached prosecretory granules reactive for CMPase and surrounded by a corona of small vesicles, and smooth-surfaced spherical CMPase-negative granules having a denser content that were identified as fully formed secretion granules; there were also occasional free trans-tubular networks strongly reactive for CMPase that appeared to undergo fragmentation and numerous small vesicles free from acid-phosphatase activity. These various images were interpreted as indicating that prosecretory granules formed in relation to two or three fenestrated saccules on the trans-side of the stack. Such granules, following their detachment from the trans-face of the stack, their separation from trans-tubular networks, and condensation of their content, yielded mature secretion granules.
利用电子显微镜照片立体对，对胰腺腺泡细胞切片中高尔基体及其组成成分的三维结构进行了分析。用戊二醛固定的胰腺组织经还原锇后固定，切片用柠檬酸铅染色。还对组织进行处理以显示磷酸酶活性（即烟酰胺腺嘌呤二核苷酸磷酸酶，NADPase；硫胺素焦磷酸酶，TPPase；胞苷单磷酸酶，CMPase）。沿着分支、吻合、连续的带状高尔基体观察到以下堆叠成分。1）在高尔基体堆叠的顺面有一个管状膜网络，已知其对锇有亲和性，称为顺面嗜锇管状网络或顺面元件。2）第一个开孔较少的囊泡，对所测试的磷酸酶无反应，在某些地方略有扩张，含有蓬松的颗粒状细丝物质。3）下面的三四个囊泡，对NADPase和/或TPPase有反应，显示出扩张部分，含有与填充囊泡其余部分相似的颗粒状细丝分泌物质。它们还显示出未扩张部分有大的开孔，其中一些开孔对齐并形成含有80纳米小泡的腔。这些囊泡的扩张部分沿囊泡长度随机分布，并非仅位于其边缘。4）堆叠中其余的一两个成分，CMPase阳性，显示出扩张的球形部分或前分泌颗粒，含有均匀的分泌物质，以及没有分泌物质且具有窄膜性小管网络外观的扁平开孔区域。5）最后，在堆叠的反面有对CMPase有反应的游离前分泌颗粒，被小泡冠包围；还有表面光滑的球形CMPase阴性颗粒，其内容物密度较大，被鉴定为完全形成的分泌颗粒；也偶尔有对CMPase强烈反应的游离反面管状网络，似乎正在发生断裂，以及许多没有酸性磷酸酶活性的小泡。这些不同的图像被解释为表明前分泌颗粒与堆叠反面的两三个开孔囊泡有关。这样的颗粒从堆叠的反面脱离、与反面管状网络分离并使其内容物浓缩后，产生成熟的分泌颗粒。
