Chen X M
Department of Nephrology, PLA General Hospital, Beijing.
Zhonghua Yi Xue Za Zhi. 1992 Nov;72(11):664-6, 702.
To investigate the relationship between Interleukin 6(IL 6) and glomerular mesangial cells (MC), the MC of BALB/c mouse were cultured and verified subsequently by anti-keratin and antidesmin antibodies. The cultured MC were divided into two parts those with Mac-1 marker and those without by flow cytometry. The IL 6 activity of cultured supernatants of MC was measured using the IL 6-dependent cell line subclone (MH 60). The results show that the mouse MC can produce IL6 and the level of IL 6 produced by MC with Mac-1 marker was higher than that without Mac-1. The RNA from the mouse glomerular mesangial cells was hybridized in dot blots with a-32pdCTP labeled IL6 cDNA probe, the dot blot autoradiograph showed the expression of IL6 mRNA gene in mouse mesangial cells.
为研究白细胞介素6（IL-6）与肾小球系膜细胞（MC）之间的关系，培养了BALB/c小鼠的系膜细胞，并随后用抗角蛋白和抗结蛋白抗体进行鉴定。通过流式细胞术将培养的系膜细胞分为有Mac-1标记的细胞和无Mac-1标记的细胞两部分。使用依赖IL-6的细胞系亚克隆（MH 60）测量系膜细胞培养上清液的IL-6活性。结果显示，小鼠系膜细胞能够产生IL-6，且有Mac-1标记的系膜细胞产生的IL-6水平高于无Mac-1标记的细胞。用α-32P-dCTP标记的IL-6 cDNA探针与小鼠肾小球系膜细胞的RNA进行斑点杂交，斑点杂交放射自显影片显示小鼠系膜细胞中有IL-6 mRNA基因的表达。
