Ikeda M, Ikeda U, Ohara T, Kusano E, Kano S
Division of Clinical Immunology, Jichi Medical School, Tochiigi, Japan.
Am J Pathol. 1992 Aug;141(2):327-34.
Murine recombinant interleukin-6 (IL-6) inhibited [3H]thymidine uptake by cultured rat mesangial cells in a dose-dependent manner in the presence of 0.5% fetal bovine serum (FBS). The inhibitory effect of IL-6 on the growth of mesangial cells was also confirmed by a change in cell numbers. In the presence of increased concentrations of FBS (5% or 10%), the effect of IL-6 was not prominent. IL-6 showed no effects on intracellular Ca2+ levels of mesangial cells. IL-6 gene expression was rapidly induced in the "quiescent" mesangial cells by exposure to 20% FBS. These observations support the premise that IL-6 is synthesized in mesangial cells and inhibits the growth of mesangial cells in an autocrine manner.
在含有0.5%胎牛血清（FBS）的情况下，小鼠重组白细胞介素-6（IL-6）以剂量依赖方式抑制培养的大鼠系膜细胞摄取[3H]胸腺嘧啶核苷。通过细胞数量的变化也证实了IL-6对系膜细胞生长的抑制作用。在FBS浓度增加（5%或10%）时，IL-6的作用不明显。IL-6对系膜细胞的细胞内Ca2+水平无影响。通过暴露于20% FBS，“静止”的系膜细胞中IL-6基因表达迅速被诱导。这些观察结果支持了IL-6在系膜细胞中合成并以自分泌方式抑制系膜细胞生长这一前提。
