Ponomarev A P, Soboleva L P, Andreeva O G, Bodina N A, Uziumov V L
Nauchnye Doki Vyss Shkoly Biol Nauki. 1992(7):66-73.
The methodological approaches of isolation of preparations of FMDV structural polypeptides to analyse them by the electrophoresis and electro-focussing methods are presented. The value of isoelectric points of protein coat of FMDV structural polypeptides and corresponding them values of electric potential are determined. The similarity and differences of FMDV serotypes, characterized by the value of relative surface, falling on separate polypeptides, are determined for the virion structure on the basis of superposition principle. FMDV has been shown to possess the summarized negative charge of different values. The charge depends on the virus type and it is a determining condition for viruses resistance in environment. A graphical model of FMDV is suggested on the basis of systemic approach and it reflects the dipole character of electric charge distribution in virion structure and agrees with the virus model, built on the basis of icosahedron symmetry.
介绍了口蹄疫病毒（FMDV）结构多肽制剂的分离方法，以便通过电泳和电聚焦方法对其进行分析。确定了FMDV结构多肽蛋白衣壳的等电点值及其相应的电势值。基于叠加原理，根据落在不同多肽上的相对表面积值，确定了FMDV各血清型在病毒粒子结构方面的异同。已证明FMDV具有不同数值的总负电荷。电荷取决于病毒类型，是病毒在环境中抗性的决定条件。基于系统方法提出了FMDV的图形模型，该模型反映了病毒粒子结构中电荷分布的偶极特性，并与基于二十面体对称性构建的病毒模型相符。
