Kolena J, Danisová A, Matejcíková K
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava.
Endocr Regul. 1992 Dec;26(4):171-4.
The role of phospholipids in the interaction of LH/hCG with membrane-bound and water-soluble receptors was studied. Digestion of porcine corpora lutea (CL) membranes with phospholipase-A2 (PL-A2) diminished, in a dose dependent manner, both phospholipid concentration and binding of 125I-hCG to CL membranes. Treatment of the water-soluble LH/hCG receptor occurring in follicular fluid with PL-A2 produced no inhibitory effect on gonadotropin binding in spite of digestion of phospholipids. The decrease of the accessibility of LH/hCG receptors in CL membranes by PL-A2 was associated with a rise of the rigidity of membrane lipids. Dynamic properties of membranes were monitored by electron spin resonance of 5- and 16-doxyl stearic acid probes. These findings suggest that phospholipids play an important role in the accessibility of membrane-bound LH/hCG receptors, but are not involved in a direct interaction of gonadotropin with the binding sites.
研究了磷脂在促黄体生成素/人绒毛膜促性腺激素（LH/hCG）与膜结合受体和水溶性受体相互作用中的作用。用磷脂酶A2（PL-A2）消化猪黄体（CL）膜，磷脂浓度和125I-hCG与CL膜的结合均呈剂量依赖性降低。尽管磷脂被消化，但用PL-A2处理卵泡液中存在的水溶性LH/hCG受体对促性腺激素结合没有产生抑制作用。PL-A2降低CL膜中LH/hCG受体的可及性与膜脂刚性增加有关。通过5-和16-二氧硬脂酸探针的电子自旋共振监测膜的动态特性。这些发现表明，磷脂在膜结合的LH/hCG受体的可及性中起重要作用，但不参与促性腺激素与结合位点的直接相互作用。
