Scsuková S, Kolena J
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovakia.
Gen Physiol Biophys. 1995 Dec;14(6):503-13.
Porcine corpora lutea plasma membranes were treated with neutral lipids from hen egg yolk and a special mixture consisting of 70% neutral lipids, 20% phosphatidylcholine and 10% phosphatidylethanolamine. Membrane fluidity, as determined by fluorescence polarization of DPH, significantly increased already after 30 min of incubation of the membranes with lipids. The fluidization effect of neutral lipids increased with the increasing concentration of lipids up to a concentration of 1 mg.ml-1. In experiments with plasma membranes labelled with [3H]cholesterol the increase in membrane fluidity was found to be partially due to a decrease of membrane cholesterol concentration. The most potent concentration of neutral lipids in extracting cholesterol was 2 mg.ml-1. The increase in fluidity of plasma membranes caused by neutral lipids and lipid mixture decreased the accessibility of the LH/hCG receptor.
用来自鸡蛋黄的中性脂质以及由70%中性脂质、20%磷脂酰胆碱和10%磷脂酰乙醇胺组成的特殊混合物处理猪黄体的质膜。通过DPH的荧光偏振测定的膜流动性，在膜与脂质孵育30分钟后就显著增加。中性脂质的流化作用随着脂质浓度增加至1mg/ml而增强。在用[3H]胆固醇标记的质膜的实验中，发现膜流动性的增加部分是由于膜胆固醇浓度的降低。提取胆固醇的中性脂质的最有效浓度为2mg/ml。中性脂质和脂质混合物引起的质膜流动性增加降低了LH/hCG受体的可及性。
