Wang M X, Earley J J, Shields J A, Donoso L A
Retina Service, Wills Eye Hospital, Philadelphia, PA 19107-5598.
Arch Ophthalmol. 1992 Mar;110(3):399-404. doi: 10.1001/archopht.1992.01080150097036.
Monoclonal antibody (MAb) 8-1H defines a highly conserved ocular melanoma-associated antigen. Based on a potential binding site of MAb8-1, we identified, molecularly cloned, and sequenced the gene, encoding this melanoma-associated antigen from a uveal melanoma copy DNA library. Nucleotide sequence analysis of the melanoma-associated antigen revealed characteristics of a membrane-bound protein of 238 amino acids (approximately 25 kd) with four transmembrane domains. The sequence of the ocular melanoma-associated antigen is identical to the sequence of a recently reported cutaneous melanoma-associated antigen, substantiating our previous studies regarding common antigens between ocular and cutaneous melanoma. In addition, the melanoma-associated antigen shares sequence homology with a number of transmembrane proteins that appear to be involved in growth regulation. The implication of the biological function of this ocular melanoma-associated antigen, its relevance to the malignant phenotype, and clinical applications of the molecular characterization of this melanoma-associated antigen are described.
单克隆抗体（MAb）8-1H可识别一种高度保守的眼部黑色素瘤相关抗原。基于MAb8-1的潜在结合位点，我们从葡萄膜黑色素瘤互补DNA文库中鉴定、分子克隆并测序了编码这种黑色素瘤相关抗原的基因。对该黑色素瘤相关抗原的核苷酸序列分析显示，它是一种具有四个跨膜结构域的238个氨基酸（约25kd）的膜结合蛋白。眼部黑色素瘤相关抗原的序列与最近报道的皮肤黑色素瘤相关抗原的序列相同，证实了我们之前关于眼部和皮肤黑色素瘤共同抗原的研究。此外，该黑色素瘤相关抗原与一些似乎参与生长调节的跨膜蛋白具有序列同源性。本文还描述了这种眼部黑色素瘤相关抗原的生物学功能的意义、其与恶性表型的相关性以及该黑色素瘤相关抗原分子特征的临床应用。
