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B染色体重复DNA序列的分子特征分析

Molecular characterization of repetitive DNA sequences from a B chromosome.

作者信息

Eickbush D G, Eickbush T H, Werren J H

机构信息

Department of Biology, University of Rochester, NY 14627.

出版信息

Chromosoma. 1992 Aug;101(9):575-83. doi: 10.1007/BF00660317.

DOI:10.1007/BF00660317
PMID:1339320
Abstract

In the parasitic wasp Nasonia vitripennis, certain males carry a B chromosome, called PSR (paternal sex ratio), which causes the compaction and subsequent loss of the paternal chromosomes in fertilized eggs. Because Nasonia are haplo-diploid, this leads to the production of all-male broods. Three families (PSR2, PSR18, PSR22) of related, tandemly repetitive DNAs were shown to be present solely on the PSR chromosome. These three families shared two conserved, palindromic DNA sequences, which may play a role in either PSR function or amplification of the tandem arrays. The tandem repeat family NV79 was determined to be present on the PSR chromosome as well as on at least one of the A chromosomes. This shared repeat as well as two repeat families (NV85, NV126) that were localized on the A chromosomes were detected in two sibling species of N. vitripennis. NV79 and NV126 were also found in the more distantly related species, Trichomalopsis dubius.

摘要

在寄生黄蜂丽蝇蛹集金小蜂(Nasonia vitripennis)中,某些雄性携带一种B染色体,称为PSR(父本性别比),它会导致受精卵中父本染色体的压缩并随后丢失。由于丽蝇蛹集金小蜂是单倍体 - 二倍体,这导致产生全是雄性的后代。已证明三个相关的串联重复DNA家族(PSR2、PSR18、PSR22)仅存在于PSR染色体上。这三个家族共享两个保守的回文DNA序列,它们可能在PSR功能或串联阵列的扩增中起作用。串联重复家族NV79被确定既存在于PSR染色体上,也存在于至少一条A染色体上。这种共享重复序列以及定位在A染色体上的两个重复家族(NV85、NV126)在丽蝇蛹集金小蜂的两个姊妹物种中被检测到。NV79和NV126在亲缘关系更远的物种—— dubius Trichomalopsis中也被发现。

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