Peczyńska-Czoch W, Zal T, Czerwiński M, Szulc M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1992;40(5-6):319-24.
A novel hemagglutinin produced by insect pathogen Beauveria bassiana was isolated from mycelium of the stationary growing microorganism and purified by adsorption on carboxymethyl cellulose followed by separation on Spherogel TSK--phenyl 5 PW column using high performance liquid chromatography. The purified hemagglutinin was homogeneous in polyacrylamide gel electrophoresis and isoelectric focusing. Its molecular weight was estimated to be around 25,000, isoelectric point was found at pH 8.6 +/- 0.2. Amino acid composition of purified B. bassiana hemagglutinin was determined by HPLC fluorometric analysis of o-phthaldialdehyde derivatives of protein hydrolysate. Purified hemagglutinin agglutinated some animal and all human erythrocytes independently of blood group ABO phenotype. The observed hemagglutination is not inhibited by glucose/mannose, N-acetylglucosamine, N-acetyl galactosamine, galactose, L-fucose and sialic acid.
从静止生长的昆虫病原球孢白僵菌菌丝体中分离出一种新型血凝素，并通过吸附于羧甲基纤维素进行纯化，随后使用高效液相色谱在Spherogel TSK-苯基5 PW柱上进行分离。纯化后的血凝素在聚丙烯酰胺凝胶电泳和等电聚焦中表现均一。其分子量估计约为25,000，等电点在pH 8.6±0.2处。通过对蛋白质水解产物的邻苯二甲醛衍生物进行HPLC荧光分析，测定了纯化的球孢白僵菌血凝素的氨基酸组成。纯化后的血凝素能凝集一些动物和所有人类红细胞，且与ABO血型表型无关。观察到的血凝反应不受葡萄糖/甘露糖、N-乙酰葡糖胺、N-乙酰半乳糖胺、半乳糖、L-岩藻糖和唾液酸的抑制。
