Rosales-Encina J L, Schlie-Guzmán M A, Jiménez-Delgadillo B, Talamás-Rohana P, Matluk M R
Departamento de Patología Experimental, CINVESTAV-IPN, México, DF.
Arch Med Res. 1992;23(2):243-8.
It is generally accepted that hydrolytic and cytolytic amebic components are involved in the pathogenic mechanisms of E. histolytica. We have now identified a lytic activity in two membrane proteins of 23.5 kDa and 25 kDa, which are able to lyse rat erythrocytes. The activity was purified from total homogenates of the virulent strains HM1:IMSS and HM38:IMSS, and the erythrocyte lysis was directly related to protein concentration. The hemolytic activity was heat-sensitive and resistant to reduction by 2-mercaptoethanol. Total amino acid analysis of pure proteins showed a high hydrophobic amino acid content: 36% for 23.5 kDa and 50% for 25 kDa. This hemolytic activity could be related, along with other amebic factors, to tissue damage.
一般认为，水解性和细胞溶解性阿米巴成分参与溶组织内阿米巴的致病机制。我们现已在两种分子量分别为23.5 kDa和25 kDa的膜蛋白中鉴定出一种裂解活性，它们能够裂解大鼠红细胞。该活性是从强毒株HM1:IMSS和HM38:IMSS的总匀浆中纯化得到的，红细胞裂解与蛋白浓度直接相关。溶血活性对热敏感，且对2-巯基乙醇的还原作用具有抗性。纯蛋白的总氨基酸分析显示，其疏水性氨基酸含量很高：23.5 kDa的蛋白为36%，25 kDa的蛋白为50%。这种溶血活性可能与其他阿米巴因素一起导致组织损伤。
