Avila E E, Liévano M A, Román M, Calderón J
Depto de biología Celular, Centro de Investigación y Estudios Avanzados IPN, México, D.F.
Arch Invest Med (Mex). 1990;21 Suppl 1:109-13.
We defined the major specificities of antibodies present in sera from patients with amebic liver abscesses, diagnosed by gamagrafia. These immune reactivities were analyzed against plasma membrane and extract antigens of Entamoeba histolytica, resolved by electrophoresis and blotted to nitrocellulose scheets. We observed that each of the 26 patients revealed a distinct pattern in the plasma membrane and in the total extract preparations. Most of these sera detected a prominent plasma membrane polypeptide of 166 kDa. We also observed that the same pattern of specificities persisted in the patients sera for six months after treatment.
我们通过免疫扩散法定义了阿米巴肝脓肿患者血清中抗体的主要特异性。针对溶组织内阿米巴的质膜和提取物抗原分析了这些免疫反应性，这些抗原经电泳分离后转印到硝酸纤维素膜上。我们观察到，26例患者中的每一例在质膜和总提取物制剂中都呈现出独特的模式。这些血清中的大多数都检测到一条突出的166 kDa质膜多肽。我们还观察到，治疗后6个月患者血清中仍保持相同的特异性模式。
