Kozlov I A, Kondrashin A A, Kononenko V A, Metelsky S T
J Bioenerg. 1976 Feb;8(1):1-7. doi: 10.1007/BF01559385.
A preparation of soluble mitochondrial ATPase (coupling factor F1) containing no gamma and delta minor subunits has been isolated. The minor-subunits-deficient F1 was found to be competent in ATP hydrolysis. However, it did not demonstrate a "coupling" effect in EDTA-submitochondrial particles. A portion of the ATPase activity of EDTA particles, stimulated by the minor-subunits-deficient F1, was insensitive to oligomycin. ATPase activity of Na+-particles was changed only slightly by this F1. It is suggested that gamma and delta subunits are necessary to form specific contacts between the F1 molecule and components of the mitochrondrial membrane.
已分离出一种不含γ和δ亚基的可溶性线粒体ATP酶（偶联因子F1）制剂。发现缺乏亚基的F1具有ATP水解能力。然而，它在EDTA-亚线粒体颗粒中未表现出“偶联”效应。缺乏亚基的F1刺激的EDTA颗粒的一部分ATP酶活性对寡霉素不敏感。这种F1对Na+颗粒的ATP酶活性影响很小。有人提出，γ和δ亚基对于在F1分子与线粒体膜成分之间形成特定接触是必需的。
