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1
Isolation of alpha-subunits of factor F1 from submitochondrial particles and the reconstitution of active ATPase from isolated alpha-subunits and beta-subunits bound to the mitochondrial membrane.从亚线粒体颗粒中分离F1因子的α亚基，并从分离的α亚基和结合在线粒体内膜上的β亚基中重建活性ATP酶。
Biochem J. 1980 Nov 15;192(2):483-8. doi: 10.1042/bj1920483.
2
Cross-reconstitution of isolated F1-ATPase from potato tuber mitochondria with F1-depleted beef heart and yeast submitochondrial particles.将马铃薯块茎线粒体中分离出的F1-ATP酶与去除F1的牛心和酵母亚线粒体颗粒进行交叉重组。
FEBS Lett. 1987 Nov 2;223(2):309-14. doi: 10.1016/0014-5793(87)80310-1.
3
Cation requirement for the reconstitution of oligomycin-sensitive ATPase by means of soluble F1-ATPase and F1-depleted submitochondrial particles.利用可溶性F1-ATP酶和去除F1的亚线粒体颗粒重组寡霉素敏感ATP酶对阳离子的需求。
Biochim Biophys Acta. 1983 Apr 22;723(1):1-6. doi: 10.1016/0005-2728(83)90002-6.
4
Titration of the binding sites for the oligomycin-sensitivity conferring protein in beef heart submitochondrial particles.牛肉心亚线粒体颗粒中寡霉素敏感性赋予蛋白结合位点的滴定
FEBS Lett. 1983 May 30;156(1):99-102. doi: 10.1016/0014-5793(83)80256-7.
5
The non-catalytic nucleotide-binding site of mitochondrial ATPase is localised on the alpha-subunit(s) of factor F1.线粒体ATP酶的非催化性核苷酸结合位点定位于F1因子的α亚基上。
Eur J Biochem. 1980 May;106(2):457-62. doi: 10.1111/j.1432-1033.1980.tb04592.x.
6
ATP synthase complex from bovine heart mitochondria. Subunit arrangement as revealed by nearest neighbor analysis and susceptibility to trypsin.牛心线粒体的ATP合酶复合体。通过最近邻分析和对胰蛋白酶的敏感性揭示的亚基排列。
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7
ATP synthase from bovine heart mitochondria: identification by proteolysis of sites in F0 exposed by removal of F1 and the oligomycin-sensitivity conferral protein.牛心线粒体ATP合酶：通过去除F1和寡霉素敏感性赋予蛋白后暴露的F0位点的蛋白水解鉴定。
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8
Resolution and reconstitution of H+ -ATPase complex from beef heart mitochondria.从牛心线粒体中解析和重组H⁺-ATP酶复合体
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Kinetics of interaction between the H+-translocating component of the mitochondrial ATPase complex and oligomycin or dicyclohexylcarbodiimide.线粒体ATP酶复合体的H⁺转运成分与寡霉素或二环己基碳二亚胺之间的相互作用动力学
Biochem Biophys Res Commun. 1983 Feb 28;111(1):333-9. doi: 10.1016/s0006-291x(83)80156-9.
10
Reconstitution of mitochondrial oligomycin and dicyclohexylcarbodiimide-sensitive ATPase.线粒体寡霉素和二环己基碳二亚胺敏感型ATP酶的重组。
Eur J Biochem. 1980 Sep;110(1):225-35. doi: 10.1111/j.1432-1033.1980.tb04859.x.

引用本文的文献

1
Photosynthetic ATPases: purification, properties, subunit isolation and function.光合 ATP 酶：纯化、性质、亚基分离与功能。
Photosynth Res. 1985 Mar;6(1):3-31. doi: 10.1007/BF00029044.
2
Topography of the subunits of Micrococcus lysodeikticus F1-ATPase.
Mol Cell Biochem. 1983;56(1):73-80. doi: 10.1007/BF00228771.

本文引用的文献

1
The non-catalytic nucleotide-binding site of mitochondrial ATPase is localised on the alpha-subunit(s) of factor F1.线粒体ATP酶的非催化性核苷酸结合位点定位于F1因子的α亚基上。
Eur J Biochem. 1980 May;106(2):457-62. doi: 10.1111/j.1432-1033.1980.tb04592.x.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
Partial resolution of the enzymes catalyzing oxidative phosphorylation. 13. Structure and function of submitochondrial particles completely resolved with respect to coupling factor.催化氧化磷酸化的酶的部分解析。13. 关于偶联因子完全解析的亚线粒体颗粒的结构与功能。
J Biol Chem. 1967 May 25;242(10):2547-51.
4
The subunit structure of beef heart mitochondrial adenosine triphosphatase. Physical and chemical properties of isolated subunits.牛心线粒体腺苷三磷酸酶的亚基结构。分离亚基的物理和化学性质。
J Biol Chem. 1972 Oct 25;247(20):6624-30.
5
Adenosine triphosphatase from rat liver mitochondria. I. Purification, homogeneity, and physical properties.
J Biol Chem. 1971 Aug 25;246(16):4987-94.
6
Partial resolution of the enzyme catalyzing oxidative phosphorylation. XXII. Interaction between mitochondrial adenosine triphosphatase inhibitor and mitochondrial adenosine triphosphatase.催化氧化磷酸化的酶的部分解离。XXII. 线粒体腺苷三磷酸酶抑制剂与线粒体腺苷三磷酸酶之间的相互作用。
J Biol Chem. 1970 Mar 25;245(6):1336-44.
7
The ATP-and ADP-binding sites in mitochondrial coupling factor F1 and their possible role in oxidative phosphorylation.线粒体偶联因子F1中的ATP和ADP结合位点及其在氧化磷酸化中的可能作用。
FEBS Lett. 1979 Jul 1;103(1):7-11. doi: 10.1016/0014-5793(79)81239-9.
8
The mitochondrial ATPase. Evidence for a single essential tyrosine residue.
Eur J Biochem. 1975 May;54(1):117-26. doi: 10.1111/j.1432-1033.1975.tb04120.x.
9
An active-site-directed adenosine triphosphate analogue binds to the beta-subunits of factor F1 mitochondrial adenosine triphosphatase with its triphosphate moiety.一种活性位点导向的三磷酸腺苷类似物通过其三磷酸部分与线粒体三磷酸腺苷酶F1因子的β亚基结合。
Biochem J. 1979 Aug 15;182(2):617-9. doi: 10.1042/bj1820617.
10
Localisation of adenine nucleotide-binding sites on beef-heart mitochondrial ATPase by photolabelling with 8-azido-ADP and 8-azido-ATP.利用8-叠氮基-ADP和8-叠氮基-ATP进行光标记定位牛肉心脏线粒体ATP酶上的腺嘌呤核苷酸结合位点
Biochim Biophys Acta. 1979 Oct 10;548(1):85-95. doi: 10.1016/0005-2728(79)90189-0.

从亚线粒体颗粒中分离F1因子的α亚基，并从分离的α亚基和结合在线粒体内膜上的β亚基中重建活性ATP酶。

Isolation of alpha-subunits of factor F1 from submitochondrial particles and the reconstitution of active ATPase from isolated alpha-subunits and beta-subunits bound to the mitochondrial membrane.

作者信息

Kozlov I A, Milgrom Y M, Tsybovski I S

出版信息

Biochem J. 1980 Nov 15;192(2):483-8. doi: 10.1042/bj1920483.

DOI:10.1042/bj1920483
PMID:6453586
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162362/
Abstract

The alpha-subunits of factor-F1 ATPase are removed by extraction of submitochondrial particles with 1.75 M-LiCl, with the consequent loss of ATPase activity. ATPase activity is reconstituted by incubation of LiCl-extracted particles with purified alpha-subunits, and the reconstituted ATPase activity is oligomycin-sensitive. Reconstitution is enhanced by maintenance of the alpha-subunits in reduced form by dithiothreitol or NaBH4 and by modification of the alpha-subunits by p-chloromercuribenzoate, iodoacetic acid or N-ethylmaleimide. Experiments with the mixed anhydride of ATP and mesitylene-carboxylic acid, which was previously shown to interact with the F1 active site, localized on the beta-subunits, indicate that the active site of ATPase is shielded by the alpha-subunits.

摘要

用1.75M-LiCl提取亚线粒体颗粒可去除F1-ATP酶的α亚基，随之ATP酶活性丧失。通过将LiCl提取的颗粒与纯化的α亚基一起温育可重建ATP酶活性，且重建的ATP酶活性对寡霉素敏感。用二硫苏糖醇或硼氢化钠将α亚基维持在还原形式，以及用对氯汞苯甲酸、碘乙酸或N-乙基马来酰亚胺修饰α亚基，均可增强重建效果。先前已证明ATP与均三甲苯羧酸的混合酸酐可与位于β亚基上的F1活性位点相互作用，对其进行的实验表明，ATP酶的活性位点被α亚基所屏蔽。