关于α-促黑素细胞激素和MIF-1对大鼠脑内环磷酸腺苷水平影响的研究。

Investigations on alpha-MSH and MIF-1 effects on cyclic AMP levels in rat brain.

作者信息

Christensen C W, Harston C T, Kastin A J, Kostrzewa R M, Spirtes M A

出版信息

Pharmacol Biochem Behav. 1976;5(Suppl 1):117-20. doi: 10.1016/0091-3057(76)90338-5.

Abstract

It has been suggested that the peptides alpha-metanocyte stimulating hormone (alpha-MSH) and MSH-release inhibitory factor (MIF-1) may alter adenosine-3', 5'-cyclic monophosphate (cAMP) metabolism [13,26]. Normal and hypophysectomized (hypoxed) rats were administered saline (controls IP daily X 3), alpha-MSH (80 mug/kg IP daily X 3) or MIF-1 (1 or 10 mg/kg IP daily X 3) and sacrificed 30 min after the third injection in a focused microwave oven (1.5 KW; 2-3 sec). Various brain areas were then assayed for cAMP levels after each treatment. The occipital cortex area was the only area to show consistent changes in both normal and hypoxed rats after alpha-MSH treatment. These findings were replicated for the occipital cortex in a second group of normal and hypoxed rats which were similarly treated. The results suggest a correlation between the rise in cAMP found and reported changes in visual acuity and attention in rats and human after treatment with alpha-MSH [8,14, 23].

摘要

有人提出，肽类α-黑素细胞刺激素（α-MSH）和促黑素释放抑制因子（MIF-1）可能会改变3'，5'-环磷酸腺苷（cAMP）的代谢[13,26]。给正常和垂体切除（hypoxed）的大鼠腹腔注射生理盐水（对照组，每日一次，共3天）、α-MSH（80微克/千克，腹腔注射，每日一次，共3天）或MIF-1（1或10毫克/千克，腹腔注射，每日一次，共3天），并在第三次注射后30分钟在聚焦微波炉（1.5千瓦；2 - 3秒）中处死。然后在每次处理后测定各个脑区的cAMP水平。枕叶皮质区域是α-MSH处理后在正常和hypoxed大鼠中唯一显示出一致变化的区域。在另一组接受类似处理的正常和hypoxed大鼠中，枕叶皮质重复了这些发现。结果表明，在用α-MSH处理后，大鼠和人类中发现的cAMP升高与视力和注意力的 reported 变化之间存在相关性[8,14,23]。