Lima L C, Massara C L, de Souza C P, Vidigal T D, Lenzi H L, Carvalho O dos S
Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, Brasil.
Rev Inst Med Trop Sao Paulo. 1992 Sep-Oct;34(5):399-402. doi: 10.1590/s0036-46651992000500005.
Biomphalaria glabrata (control), B. tenagophila and B. straminea from our laboratory colonies iniciated with molluscs collected in the municipality of Belo Horizonte, MG (Brasil), were experimentally infected with first-stage larvae of Angiostrongylus costaricensis. The number of molluscs of each species exposed was 139, 77 and 149. About 25 days later, surviving molluscs were individually examined by artificial digestion. Of 87 B. glabrata examined, 62 (71.3%) were positive and between one and 61 third-stage larvae were found; of 42 B. tenagophila, 21 (50.0%) contained between one five third-stage larvae; and of 89 B. straminea, 69 (77.5%) presented between one and 72 third-stage larvae. The three molluscan species are susceptible to A. costaricensis infection, but B. glabrata and B. straminea are most suitable for maintaining the nematode cycle in laboratory.
来自我们实验室种群的光滑双脐螺（对照）、嗜气管双脐螺和淡黄双脐螺，这些种群最初是用在巴西米纳斯吉拉斯州贝洛奥里藏特市采集的软体动物建立的，用哥斯达黎加管圆线虫的一期幼虫进行了实验性感染。每个物种暴露的软体动物数量分别为139只、77只和149只。大约25天后，对存活的软体动物进行人工消化单独检查。在检查的87只光滑双脐螺中，62只（71.3%）呈阳性，发现1至61条三期幼虫；在42只嗜气管双脐螺中，21只（50.0%）含有1至5条三期幼虫；在89只淡黄双脐螺中，69只（77.5%）有1至72条三期幼虫。这三种软体动物物种都易受哥斯达黎加管圆线虫感染，但光滑双脐螺和淡黄双脐螺最适合在实验室维持线虫循环。
