Kronester-Frei A
Laryngol Rhinol Otol (Stuttg). 1976 Aug;55(8):687-700.
To date the structure of the marginal net of the tectorial membrane has not been sufficiently described, its function is unclear and can only be understood after further detailed analysis. The limiting factor in these experiments is that mostly the applied routine preparation techniques always unspecifically change the tectorial membrane. The preparation techniques have now been modified so far that the induced changes can be kept at a minimum. Examinations were carried out parallely with the stereo microscope, the light microscope, the scanning and transmission electron microscopes; results were compared and ensured. 1. The normal structure of the marginal net of the adult squirrel monkey was compared with that of the adult rabbit. The similarity suggests to study for the first time the development of the marginal net in the embryologically more easily accessable rabbit by means of scanning electron microscope. 2. The refined preparation technique enables a reproducable investigation of the tectorial membrane in a lifted off state and subsequently in a large area even in a not lifted off state. 3. During the various steps of preparation the marginal zone is deformed much stronger than the rest of the membrane. 4. In the squirrel monkey a distinctly developed marginal net seems to be combined with the presence of a fourth row of other hair cells. To this conclusion leads the following observation: the impressions of the sensory cell hairs on the bottom side of the tectorial membrane are found predominately in the cross connections of the marginal net. 5. The marginal net of the rabbit shows a development from basal to apical. Thereby the hair cells are covered with fibres of the tectorial membrane up to the Hensen's cells. 6. On the fifth day characteristic holes are growing in the marginal area and the fibres withdraw from the Hensen' cells. This development proceeds in the rabbit more than in the squirrel monkey, the emerged net structure is reduced to the advantage of the marginal band. 7. A new technique allows the same spot to be observed by the scanning as well as the transmission electron microscopes. The interdental cells examinated in this way showed a strong activity of synthesis in the "3d" stage.
迄今为止，盖膜边缘网络的结构尚未得到充分描述，其功能尚不清楚，只有经过进一步详细分析才能理解。这些实验中的限制因素在于，大多数应用的常规制备技术总是非特异性地改变盖膜。目前制备技术已得到改进，使得诱导的变化能够保持在最低限度。使用体视显微镜、光学显微镜、扫描电子显微镜和透射电子显微镜进行了平行检查；对结果进行了比较和验证。1. 将成年松鼠猴盖膜边缘网络的正常结构与成年兔子的进行了比较。这种相似性表明首次通过扫描电子显微镜研究胚胎学上更容易操作的兔子盖膜边缘网络的发育情况。2. 改进后的制备技术能够对处于掀起状态以及随后即使在未掀起状态下的大面积盖膜进行可重复的研究。3. 在制备的各个步骤中，边缘区域的变形比膜的其余部分要强得多。4. 在松鼠猴中，明显发达的边缘网络似乎与第四排其他毛细胞的存在有关。以下观察结果得出了这一结论：感觉细胞毛在盖膜底面的印记主要出现在边缘网络的交叉连接中。5. 兔子的盖膜边缘网络显示出从基部到顶端的发育过程。由此，毛细胞被盖膜纤维覆盖直至亨森细胞。6. 在第五天，边缘区域出现了特征性的孔洞，纤维从亨森细胞中撤出。这种发育在兔子中比在松鼠猴中更为明显，出现的网络结构向边缘带的方向减少。7. 一种新技术允许用扫描电子显微镜和透射电子显微镜观察同一点。以这种方式检查的齿间细胞在“3d”阶段显示出强烈的合成活性。
