Haidar M A
Philos Trans R Soc Lond B Biol Sci. 1976 Nov 30;276(943):165-72. doi: 10.1098/rstb.1976.0107.
For the purpose of attempting to generalize the rules concerning morphogenesis of helical viruses, the in vitro reconstitution of the CAM strain of TRV was studied. The conditions for reconstitution and the importance of the aggregation state of the protein for initiation and elongation are compared with those of TMV. The initiation step consisting of the binding of RNA with the 36S disk of protein was easily accomplished. The polarity and the specificity of encapsidation of TRV RNA by homologous and heterologous viral protein is discussed.
为了尝试归纳有关螺旋病毒形态发生的规则，对烟草脆裂病毒（TRV）CAM株系进行了体外重建研究。将重建条件以及蛋白质聚集状态对起始和延伸的重要性与烟草花叶病毒（TMV）的相关条件进行了比较。由RNA与36S蛋白质盘结合组成的起始步骤很容易完成。还讨论了同源和异源病毒蛋白对TRV RNA衣壳化的极性和特异性。
