Willis L G, Winston M L, Honda B M
Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.
Mol Phylogenet Evol. 1992 Sep;1(3):169-78. doi: 10.1016/1055-7903(92)90013-7.
The complete nucleotide sequence of the mitochondrial cytochrome oxidase II (COII) gene was determined for five species of the honeybee (Genus: Apis): A. andreniformis, A. cerana, A. dorsata, A. florea, and A. koschevnikovi; these were then compared to the known sequence of the A. millifera gene from Crozier et al. (1989, Mol. Biol. Evol., 6: 399-411) and the wasp Excristes roborator (Liu and Beckenbach, 1992, Mol. Phylogenet. Evol., 1:41-52). Phylogenetic relationships were derived using the parasimony methods DNAPARS and PROTPARS of Felsenstein ("PHYLIP Manual Version 3.4, "University Herbarium, Univ. of California, Berkeley). The results suggest that A. dorsata is the most ancestral species, followed by the branching of A. florea/A. andreniformis and A. koschevnikovi, and then A. mellifera and A. cerana. This inference differs from the currently accepted view that considers the A. florea/A. andreniformis line to be the most ancestral.
测定了蜜蜂属（Apis）5个物种的线粒体细胞色素氧化酶II（COII）基因的完整核苷酸序列，这5个物种分别是：黑小蜜蜂（A. andreniformis）、中华蜜蜂（A. cerana）、大蜜蜂（A. dorsata）、小蜜蜂（A. florea）和科氏蜜蜂（A. koschevnikovi）；然后将这些序列与克罗齐尔等人（1989年，《分子生物学与进化》，6: 399 - 411）提供的西方蜜蜂（A. millifera）基因已知序列以及黄蜂（Excristes roborator）（刘和贝肯巴赫，1992年，《分子系统发育与进化》，1:41 - 52）的序列进行比较。系统发育关系采用费尔森斯坦的简约法DNAPARS和PROTPARS推导得出（《PHYLIP手册版本3.4》，加利福尼亚大学伯克利分校植物标本馆）。结果表明，大蜜蜂是最原始的物种，其次是小蜜蜂/黑小蜜蜂和科氏蜜蜂分支，然后是西方蜜蜂和中华蜜蜂。这一推断与目前公认的观点不同，目前的观点认为小蜜蜂/黑小蜜蜂谱系是最原始的。
