Pedersen B V
Department of Population Biology, University of Copenhagen, Denmark.
Mol Phylogenet Evol. 1996 Apr;5(2):289-97. doi: 10.1006/mpev.1996.0024.
PCR amplification and direct sequencing of a 532-bp region of the mt CO-1 (cytochrome oxidase I) gene from five true bumblebee species and six cuckoo bumblebee species were performed. The sequences were then aligned to the corresponding sequence in the honey bee. Phylogenetic analyses based on parsimony and maximum likelihood indicate that the cuckoo bumblebees form a monophyletic group within the true bumblebees. The Bombus lucorum group (subgenus Bombus) falls out as the sister group to a clade comprising the cuckoo bumblebees and the remaining true bumblebees. A strong A + T bias (A + T = 75%) was recorded. Comparisons of all the sequences show a high-transversion bias (A<-->T) even among closely related species. The bias is most pronounced in third positions.
对5种真熊蜂物种和6种杜鹃熊蜂物种的线粒体细胞色素氧化酶I（mt CO-1）基因的532bp区域进行了PCR扩增和直接测序。然后将这些序列与蜜蜂中的相应序列进行比对。基于简约法和最大似然法的系统发育分析表明，杜鹃熊蜂在真熊蜂中形成一个单系类群。明亮熊蜂组（熊蜂亚属）作为包括杜鹃熊蜂和其余真熊蜂的一个分支的姐妹群分支出来。记录到强烈的A+T偏向（A+T = 75%）。所有序列的比较显示，即使在亲缘关系密切的物种之间也存在较高的颠换偏向（A<-->T）。这种偏向在第三位最为明显。
