McCarthy K P, Sloane J P, Kabarowski J H, Matutes E, Wiedemann L M
Leukaemia Research Fund Centre, Institute of Cancer Research, Chester Beatty Laboratories, London, England.
Diagn Mol Pathol. 1992 Sep;1(3):173-9.
A series of T-cell proliferations in peripheral blood, bone marrow, or tissue samples, together with seven T-cell lines, were analysed for clonality. The technique used employs the polymerase chain reaction (PCR) to amplify rearranged T-cell receptor gamma genes, using primers recognising conserved sequences in the variable and joining gene segments. Of the 20 cases of T-cell leukaemia or lymphoma analysed, a clone was detected in 14 (70%): Of seven T-cell lines, a clone was detected in 6 (84%). No positive results were recorded in eight non-T-cell disorders (including nonlymphoid malignancies and reactive disorders). When the results of this technique were combined with the results of our previously published method for the detection of clonally rearranged T-cell receptor-beta (TCR-beta) genes using PCR, 9 of 10 (90%) T-cell tumours were detected. This method uses only four primer combinations in two tubes, and is therefore simple and rapid: it requires no radiolabelling, uses only a small amount of tissue, and can be performed on formalin-fixed, paraffin-embedded tissue.
对外周血、骨髓或组织样本中的一系列T细胞增殖以及7个T细胞系进行克隆性分析。所采用的技术利用聚合酶链反应（PCR），使用识别可变基因片段和连接基因片段中保守序列的引物来扩增重排的T细胞受体γ基因。在分析的20例T细胞白血病或淋巴瘤病例中，14例（70%）检测到克隆：在7个T细胞系中，6个（84%）检测到克隆。8例非T细胞疾病（包括非淋巴恶性肿瘤和反应性疾病）未记录到阳性结果。当该技术的结果与我们之前发表的使用PCR检测克隆性重排的T细胞受体β（TCR-β）基因的方法的结果相结合时，10例T细胞肿瘤中有9例（90%）被检测到。该方法仅在两个管中使用四种引物组合，因此简单快速：无需放射性标记，仅使用少量组织，并且可在福尔马林固定、石蜡包埋的组织上进行。
