Plewka A, Plewka D, Bienioszek M
II Katedry Histologii i Embriologii, Slaskiej Akademii Medycznej, Katowicach.
Folia Med Cracov. 1992;33(1-4):133-40.
This experiments were carried out on: 0.5-, 1-, 2-, 4-, 8-, 12-, 20- and 28-months old Wistar males rats. Before they were killed the animals received by intraperitoneal injection: phenobarbital (50 mg/kg in 0.9% saline 72- and 48-hr before the death), beta-naphthoflavone (20 mg/kg in sesame oil for 3 consecutive days prior to sacrifice), dexamethasone (10 mg/kg; see beta-naphthoflavone). All animals were decapitated after 24 hr of starvation. The livers were rinsed with cold 0.9% saline. Hepatic microsomes were prepared as described Dallner. The UDP-glucuronyltransferase activities with p-nitrophenol as aglycone were determined according to Burchell and Weatherill and protein concentration was measured as described Lowry et al. The activity of examined enzyme had maximal value in youngest rats, but in older one it was decreased. Minimum was observed in 8-month old animals and all older. Pretreatment of rats with beta-naphthoflavone significantly increased glucuronidation especially in sexually mature animals. The date obtained with phenobarbital and dexamethasone pretreatment rats suggested lower magnitude of stimulation of UDP-glucuronyltransferase than beta-naphthoflavone and lack of effect respectively.
这些实验是在0.5、1、2、4、8、12、20和28月龄的雄性Wistar大鼠身上进行的。在处死前，动物通过腹腔注射接受：苯巴比妥（在死亡前72小时和48小时，以50毫克/千克溶于0.9%盐水中）、β-萘黄酮（在处死前连续3天，以20毫克/千克溶于芝麻油中）、地塞米松（10毫克/千克；同β-萘黄酮）。所有动物在饥饿24小时后断头。肝脏用冷的0.9%盐水冲洗。按照达尔纳的方法制备肝微粒体。以对硝基苯酚为苷元的UDP-葡萄糖醛酸基转移酶活性根据伯切尔和韦瑟里尔的方法测定，蛋白质浓度按照洛瑞等人的方法测定。所检测酶的活性在最年幼的大鼠中具有最大值，但在年长的大鼠中降低。在8月龄及所有年长动物中观察到最小值。用β-萘黄酮预处理大鼠显著增加了葡萄糖醛酸化，尤其是在性成熟动物中。用苯巴比妥和地塞米松预处理大鼠得到的数据分别表明，UDP-葡萄糖醛酸基转移酶的刺激程度低于β-萘黄酮且无作用。
