Boron-Kaczmarska A, Hryniewicz A, Kemona A, Szabados A, Puch U, Chrostek L, Szmitkowski M
Department of Clinical Physiology, Max von Pettenkofer Institute for Hygiene and Medicine Microbiology, Ludwig-Maximillians-University, Munich, Germany.
Acta Physiol Hung. 1992;79(4):399-408.
The morphological and biochemical changes of the liver after endotoxin intake were analyzed in rats receiving 20% ethanol during 60 days. Besides morphological changes, concentration of serotonin and histamine in liver homogenates, the activity of asparagine and alanine aminotransferases (AspAT, ALAT), gamma-glutamyltranspeptidase (GGTP) and alcohol dehydrogenase (ADH) in blood serum were determined, too. The most extensive morphologic changes of the liver were seen in group of animals intoxicated with 20% ethanol during 60 days and single dose of endotoxin E. coli 0127:B8 intraperitoneally. These changes included necrosis most hepatocytes, focal steatosis of liver parenchyma, considerable hyperemia and parenchymatous degeneration of the liver cells. The cells lining liver sinuses showed considerable swelling as well as necrotic changes. Figures of cell division and haemorrhagic focuses were seen, too. The clusters of mononuclear cells, surrounding necrotically changed hepatocytes were seen in the central part of the liver lobule. Among the inflammatory mediators estimated in liver homogenate only serotonin reached a high level in the group of experimental animals receiving only endotoxin. Increased activity of aminotransferases AspAt and ALAT were associated with these morphologic and biochemical changes in liver tissue observed in animals receiving ethanol and endotoxin.
对连续60天摄入20%乙醇的大鼠内毒素摄入后肝脏的形态学和生化变化进行了分析。除了形态学变化外，还测定了肝匀浆中血清素和组胺的浓度、血清中天冬酰胺和丙氨酸转氨酶（AspAT、ALAT）、γ-谷氨酰转肽酶（GGTP）和乙醇脱氢酶（ADH）的活性。在连续60天摄入20%乙醇并腹腔注射单剂量内毒素大肠杆菌0127:B8的动物组中，观察到肝脏最广泛的形态学变化。这些变化包括大多数肝细胞坏死、肝实质局灶性脂肪变性、明显的充血和肝细胞实质变性。肝窦内衬细胞显示出明显肿胀以及坏死变化。也可见细胞分裂图像和出血灶。在肝小叶中央部分可见围绕坏死性改变肝细胞的单核细胞簇。在仅接受内毒素的实验动物组中，在肝匀浆中评估的炎症介质中，只有血清素达到高水平。在接受乙醇和内毒素的动物中观察到的肝脏组织的这些形态学和生化变化与转氨酶AspAt和ALAT活性增加有关。
