Boron-Kaczmarska A, Kozlowska H, Swiderska J, Szmitkowski M, Szymanski J
Klinikai Elettani Intézet, Biokémiai Diagnosztikai Intézet, Orvosi Akadémia, Bialystok, Lengyelország.
Orv Hetil. 1994 Jul 31;135(31):1705-8.
Endotoxin concentrations were measured in the portal, hepatic venous blood and abdominal aorta of six groups of rats in experimental alcoholic liver injury. Groups of animal consisted of 16 rats: I.: control group; II.: rats received one dose of E. coli 0127: B8-3 mg/kg intraperitoneally (i.p.), III.: rats received three doses of E. coli; IV.: rats intoxicated with 20% ethanol ad libitum (60 days); V.: rats intoxicated with one dose of endotoxin after 20% ethanol ingestion; VI.: animal intoxicated with 20% ethanol and three doses of E. coli 0127: B8. The highest levels of endotoxin (determined by Limulus Lysate test) were in portal venous blood--3.1 pg/ml in control group to 113.4 pg/ml in VI. group. The level of endotoxin in plasma hepatic venous blood was elevated from 0.0 pg/ml (I., II. groups) to 15.8 pg/ml (III.), 34.3 pg/ml (IV.), 49.7 pg/ml (V.) up to 75.1 pg/ml (VI.). Plasma endotoxin concentration in abdominal aorta was highest in group VI.--32.2 pg/ml. The percentage of excretion rate of endotoxin by liver function was measured on the basis of difference between plasma endotoxin levels in the prehepatic blood (portal vein) and posthepatic blood (hepatic vein). The highest activity of biochemical parameters indicating liver injury was observed in animals intoxicated with ethanol and endotoxin.
在实验性酒精性肝损伤的六组大鼠中，测定了门静脉、肝静脉血和腹主动脉中的内毒素浓度。每组动物有16只大鼠：I组：对照组；II组：大鼠腹腔注射一剂大肠杆菌0127:B8（3毫克/千克）；III组：大鼠接受三剂大肠杆菌；IV组：大鼠自由饮用20%乙醇（60天）；V组：大鼠在摄入20%乙醇后注射一剂内毒素；VI组：大鼠摄入20%乙醇并接受三剂大肠杆菌0127:B8。内毒素水平最高（通过鲎试剂法测定）的是门静脉血——对照组为3.1皮克/毫升，VI组为113.4皮克/毫升。肝静脉血浆中的内毒素水平从0.0皮克/毫升（I组、II组）升高到15.8皮克/毫升（III组）、34.3皮克/毫升（IV组）、49.7皮克/毫升（V组），直至75.1皮克/毫升（VI组）。腹主动脉血浆内毒素浓度在VI组最高——为32.2皮克/毫升。根据肝前血（门静脉）和肝后血（肝静脉）中血浆内毒素水平的差异，测定肝功能对内毒素的排泄率百分比。在摄入乙醇和内毒素的动物中，观察到表明肝损伤的生化参数活性最高。
