Pujol F H, Rodríguez I, Liprandi F
Gerencia Nacional de Biotecnología, Polar C.A., Venezuela.
Acta Cient Venez. 1992;43(4):235-9.
Hepatitis B virus surface antigen (HBsAg) found in a commercial vaccine was use as immunogen and antigen for the production and selection of murine monoclonal antibodies against this viral antigen. Antibody relative avidity was determined based on their capture capacity. Competition studies, and the differential recognition pattern of vaccine preparations showed that high avidity IgG1 antibodies were directed against two distinct antigenic regions. Among them, 6F4 was most suitable for the detection of HBsAg in a sandwich ELISA system, using an IgM antibody, 5E8, as capture. However, the combined use of 6F4 with another which does not recognize the same epitope did not improve the sensitivity of the assay. On the other hand, the combination of 6F4 with a lower avidity IgG1 antibody, 6G10, increased the universe of antigenic diversities recognized by this system. An enzyme immunoassay for detection of HBsAg is proposed, using 3 monoclonal antibodies: one IgM as capture and two IgG1 for detection.
一种商用疫苗中发现的乙型肝炎病毒表面抗原（HBsAg）被用作免疫原和抗原，用于生产和筛选针对该病毒抗原的鼠单克隆抗体。基于抗体的捕获能力测定其相对亲和力。竞争研究以及疫苗制剂的差异识别模式表明，高亲和力IgG1抗体针对两个不同的抗原区域。其中，6F4最适合在夹心ELISA系统中检测HBsAg，使用IgM抗体5E8作为捕获抗体。然而，将6F4与另一种不识别相同表位的抗体联合使用并不能提高检测的灵敏度。另一方面，6F4与低亲和力IgG1抗体6G10联合使用，增加了该系统识别的抗原多样性范围。提出了一种使用3种单克隆抗体检测HBsAg的酶免疫测定法：一种IgM作为捕获抗体，两种IgG1用于检测。
