Hlozánek I, Dostálová V, Korec E, Zelený V, König J, Nĕmecek V
Folia Biol (Praha). 1986;32(3):167-77.
Hybridomas secreting anti-HBsAg antibodies were produced by fusion of the mouse myeloma cell line SP2/0 with lymphocytes from mice immunized with purified HBsAg. All clones produced antibodies of the IgG1 idiotype that react with the subtype a determinant of HBsAg. An enzyme immunoassay for detection of HBsAg in human sera using monoclonal antibodies was developed and compared with commercial Sevatest ELISA HBsAg/micro I kit for detection of HBsAg in clinical serum samples.
通过将小鼠骨髓瘤细胞系SP2/0与用纯化的乙肝表面抗原（HBsAg）免疫的小鼠淋巴细胞融合，产生了分泌抗HBsAg抗体的杂交瘤。所有克隆产生的IgG1同种型抗体均与HBsAg的a亚型决定簇发生反应。开发了一种使用单克隆抗体检测人血清中HBsAg的酶免疫测定法，并与用于检测临床血清样本中HBsAg的商业Sevatest ELISA HBsAg/micro I试剂盒进行了比较。
