Mellati A A, Yücel M, Altinörs N, Gündüz U
Biology Department, Middle East Technical University, Ankara, Turkey.
Cancer Biochem Biophys. 1992 Oct;13(1):33-41.
In the present study the mechanism of action of M2-type pyruvate kinase from human meningioma in the simultaneous presence of fructose 1,6 diphosphate and L-alanine was investigated. Purified pyruvate kinase from human meningioma was allosterically inhibited by L-alanine with respect to substrates phosphoenolpyruvate and ADP. The inhibitory effects of L-alanine was partially removed by fructose 1,6 diphosphate. The purified enzyme was slightly susceptible to ATP inhibition.
在本研究中，对人脑膜瘤中M2型丙酮酸激酶在同时存在1,6-二磷酸果糖和L-丙氨酸时的作用机制进行了研究。来自人脑膜瘤的纯化丙酮酸激酶相对于底物磷酸烯醇丙酮酸和ADP受到L-丙氨酸的变构抑制。L-丙氨酸的抑制作用被1,6-二磷酸果糖部分消除。纯化的酶对ATP抑制稍有敏感性。
