体外培养动物组织的营养。IV. 鸡胚心脏成纤维细胞的氨基酸需求
The nutrition of animal tissues cultivated in vitro. IV. Amino acid requirements of chick embryonic heart fibroblasts.
作者信息
MORGAN J F, MORTON H J
出版信息
J Biophys Biochem Cytol. 1957 Mar 25;3(2):141-50. doi: 10.1083/jcb.3.2.141.
Abstract
- The amino acid requirements of freshly explanted chick embryonic heart tissues cultivated in completely synthetic media have been determined, employing a nutritional depletion technique. Arginine, histidine, lysine, tyrosine, tryptophan, phenylalanine, cystine, methionine, threonine, leucine, and valine were found to be essential. Serine, isoleucine, glycine, and glutamine were found to be non-essential. Glutamic acid, aspartic acid, alpha-alanine, proline, and hydroxyproline were found to be inhibitory in this test system. 2. A total amino acid level of approximately 100 mg. per cent was found to be optimal and DL-amino acids were found to be non-toxic, unless used in high concentrations. 3. A comparison has been made of the amino acid requirements of various types of tissue cultures, of the chick, and of man and certain differences in these requirements have been discussed.
摘要
- 采用营养耗竭技术，已确定了在完全合成培养基中培养的新鲜移植鸡胚心脏组织的氨基酸需求。发现精氨酸、组氨酸、赖氨酸、酪氨酸、色氨酸、苯丙氨酸、胱氨酸、蛋氨酸、苏氨酸、亮氨酸和缬氨酸是必需的。发现丝氨酸、异亮氨酸、甘氨酸和谷氨酰胺是非必需的。在该测试系统中，发现谷氨酸、天冬氨酸、α-丙氨酸、脯氨酸和羟脯氨酸具有抑制作用。2. 发现总氨基酸水平约为每百毫升100毫克是最佳的，并且发现DL-氨基酸无毒，除非高浓度使用。3. 已对鸡、人和某些不同类型组织培养物的氨基酸需求进行了比较，并讨论了这些需求中的某些差异。
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本文引用的文献
1
Relation of oxygen and temperature in the preservation of tissues by refrigeration.
Proc Soc Exp Biol Med. 1949 Jun;71(2):196-200. doi: 10.3181/00379727-71-17131.
2
Nutrition of animal cells in tissue culture; initial studies on a synthetic medium.
Proc Soc Exp Biol Med. 1950 Jan;73(1):1-8. doi: 10.3181/00379727-73-17557.
3
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
4
The specific amino acid requirements of a human carcinoma cell (Stain HeLa) in tissue culture.
J Exp Med. 1955 Jul 1;102(1):37-48. doi: 10.1084/jem.102.1.37.
5
The specific amino acid requirements of a mammalian cell (strain L) in tissue culture.
J Biol Chem. 1955 Jun;214(2):839-52.
6
The nutrition of animal tissues cultivated in vitro. III. Use of a depletion technique for determining specific nutritional requirements.
J Biophys Biochem Cytol. 1956 Sep 25;2(5):589-96. doi: 10.1083/jcb.2.5.589.
7
The amino acid requirements of the Walker carcinosarcoma 256 in vitro.
Cancer Res. 1956 Nov;16(10 Part 1):979-84.
8
9
The nutrition of animal tissues cultivated in vitro. II. A comparison of various synthetic media.
J Natl Cancer Inst. 1956 Jun;16(6):1405-15.
10
The growth response of mammalian cells in tissue culture to L-glutamine and L-glutamic acid.
J Biol Chem. 1956 Feb;218(2):607-16.
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