Laboratoire des Sciences du Génie Chimique - CNRS-ENSAIA, Institut National Polytechnique de Lorraine (INPL), 2, Avenue de la Forêt de Haye, F-54505, Vandoeuvre-lès-Nancy, France,
Cytotechnology. 2001 Oct;37(2):93-105. doi: 10.1023/A:1019908310300.
In the present study an approach has been developed in order to examine the consequence of essential and non essential amino acid supplementation on VO208 hybridoma cells behaviour. The effect of amino acid enrichment has been studied taking into account the culture process, i.e., batch or continuous culture mode and the medium composition, i.e., a home made serum-free medium or a serum containing one. A group of 4 amino acids, i.e., Ser, Pro, Gly and Arg presented atypical evolution pattern of their extracellular concentration depending on the type of the medium and on the culture mode. Some amino acids were probably involved in the limitation of the cellular proliferation. Met was one of the amino acids that appears to may have been at limiting concentration in all cases. In continuous culture mode, an enrichment of amino acids resulted in a rapid improvement of the viable cell density in both media, with or without the presence of serum. For most amino acids, supplementation during continuous culture induced an increase of the amino acid uptake rate. A comparative analysis of amino acids utilisation, depending on the culture conditions studied in the present study, has been performed in order to propose an overall picture of amino acids metabolism by VO 208 Hybridoma cell line.
在本研究中，我们提出了一种方法，以研究必需和非必需氨基酸补充对 VO208 杂交瘤细胞行为的影响。我们考虑了培养过程（即分批或连续培养模式）和培养基组成（即无血清自制培养基或含血清培养基）来研究氨基酸的富集效应。一组 4 种氨基酸（丝氨酸、脯氨酸、甘氨酸和精氨酸）的细胞外浓度表现出非典型的变化模式，这取决于培养基的类型和培养模式。一些氨基酸可能参与了细胞增殖的限制。甲硫氨酸是一种似乎在所有情况下都处于限制浓度的氨基酸。在连续培养模式下，无论是否含有血清，氨基酸的富集都会导致两种培养基中活细胞密度的快速提高。对于大多数氨基酸，在连续培养过程中进行补充会导致氨基酸摄取率的增加。我们根据本研究中研究的培养条件对氨基酸的利用进行了比较分析，以提出 VO208 杂交瘤细胞系的氨基酸代谢的整体情况。
