组织培养中产生的粘多糖。
Mucopolysaccharides produced in tissue culture.
作者信息
GROSSFELD H, MEYER K, GODMAN G, LINKER A
出版信息
J Biophys Biochem Cytol. 1957 May 25;3(3):391-6. doi: 10.1083/jcb.3.3.391.
Abstract
- A method of mass tissue culture has been devised by which, in a relatively short period of time, samples large enough for chemical isolation of mucopolysaccharides can be obtained. 2. Chemical isolation of acid mucopolysaccharides from mass cultures of human fetal skin, human fetal bone, bovine fetal skin, and rat subcutaneous tissue has been carried out. It has been found that the fibroblasts of each of these tissues produce in tissue culture more than one mucopolysaccharide, namely, hyaluronic acid, and a chondroitin sulfate. 3. The chondroitin sulfate produced by fibroblasts of the above tissues in tissue culture was not fully sulfated. The possible significance of this finding is discussed.
摘要
- 已经设计出一种大规模组织培养方法，通过该方法可在相对较短的时间内获得足够大的样本，用于化学分离粘多糖。2. 已经从人胎儿皮肤、人胎儿骨骼、牛胎儿皮肤和大鼠皮下组织的大规模培养物中进行了酸性粘多糖的化学分离。已经发现，这些组织中的每一种的成纤维细胞在组织培养中产生不止一种粘多糖，即透明质酸和一种硫酸软骨素。3. 上述组织的成纤维细胞在组织培养中产生的硫酸软骨素没有完全硫酸化。讨论了这一发现可能的意义。
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