Giotaki H A, Zioga C P, Ioachim-Velogianni E E
Department of Internal Medicine, General Hospital of Ioannina, Greece.
Sarcoidosis. 1992 Mar;9(1):35-8.
The phenotype of infiltrating and residual cells in biopsies of minor salivary glands from patients with sarcoidosis and from normal controls, were studied using a sensitive two-step indirect immunoperoxidase technique. We found that infiltrating lymphocytes were practically absent from salivary gland biopsies in the control group, but that in the Sarcoid group the numerous infiltrating lymphocytes consisted mainly of T-lymphocytes, mostly T-helper cells (CD4+ cells). These data correlate positively with other studies on lymphocytic infiltration in other organs involved in the immunopathology of sarcoidosis, e.g. the lung. In our study the glandular epithelial cells in the salivary gland biopsies were HLA-DR positive in the patients with sarcoidosis, especially in those specimens from patients with active disease in which 50-80% of the epithelial cells expressed an HLA-DR antigen. We observed a positive relationship between HLA-DR positive epithelial cells, the expression of interleukin-2 receptors (IL-2R) and the density of lymphocytic infiltrates. This is in accordance with other chronic, lymphocytic infiltratory diseases with HLA-DR positive epithelial expression, such as Sjögren's syndrome involving salivary glands. This suggests that there might be a common denominator causing disease in these cases.
采用灵敏的两步间接免疫过氧化物酶技术，对结节病患者和正常对照者小唾液腺活检组织中浸润细胞和残留细胞的表型进行了研究。我们发现，对照组唾液腺活检组织中几乎没有浸润淋巴细胞，但在结节病组中，大量浸润淋巴细胞主要由T淋巴细胞组成，大部分是辅助性T细胞（CD4 +细胞）。这些数据与关于结节病免疫病理学中其他受累器官（如肺）淋巴细胞浸润的其他研究呈正相关。在我们的研究中，结节病患者唾液腺活检组织中的腺上皮细胞HLA - DR呈阳性，尤其是那些来自活动性疾病患者的标本，其中50 - 80%的上皮细胞表达HLA - DR抗原。我们观察到HLA - DR阳性上皮细胞、白细胞介素 - 2受体（IL - 2R）的表达与淋巴细胞浸润密度之间存在正相关。这与其他具有HLA - DR阳性上皮表达的慢性淋巴细胞浸润性疾病一致，如累及唾液腺的干燥综合征。这表明在这些病例中可能存在导致疾病的共同因素。
