Hayek A, Beattie G M
Lucy Thorne Whittier Children's Center, Whittier Institute, La Jolla, CA 92037.
Cell Transplant. 1992;1(1):83-5. doi: 10.1177/096368979200100112.
This study was undertaken to evaluate the safety and effectiveness of intravenous (i.v.) injection of islets, via a peripheral vein, in the treatment of experimental diabetes in highly inbred Lewis rats. Rats were made diabetic by the i.v. injection of streptozocin, and donor islets were isolated from neonatal rats. Two wk after the induction of diabetes, rats with glucose concentrations > 19 mM were divided into two groups: one group of six rats was injected with 2000 neonatal islets into the external jugular vein; the second group of rats, acting as control, was injected in the same vein with medium alone. Within 2 wk of the islet transplant, 100% (6/6) of the rats were cured of the diabetes. None of the control rats experienced any improvement. As expected, pancreatic islets so administered embolized to the lungs. Glucose tolerance tests in the rats receiving the islet transplant were indistinguishable from normal rats. These results could be important to clinical transplantation if the procedure is as successful in larger animals using autotransplanted islets or allografts protected from immune rejection.
本研究旨在评估经外周静脉静脉注射胰岛治疗高度近交系Lewis大鼠实验性糖尿病的安全性和有效性。通过静脉注射链脲佐菌素使大鼠患糖尿病，并从新生大鼠中分离供体胰岛。糖尿病诱导两周后，血糖浓度>19 mM的大鼠被分为两组：一组六只大鼠经颈外静脉注射2000个新生胰岛；第二组大鼠作为对照，仅经同一静脉注射培养基。在胰岛移植的两周内，100%（6/6）的大鼠糖尿病得到治愈。对照大鼠均未出现任何改善。正如预期的那样，如此给药的胰岛栓塞到肺部。接受胰岛移植的大鼠的葡萄糖耐量试验与正常大鼠无异。如果该程序在使用自体移植胰岛或免受免疫排斥的同种异体移植物的大型动物中同样成功，这些结果对临床移植可能具有重要意义。
