Sueyoshi S, Nagakura H, Kato A, Uetsuki S, Nakayama Y, Adachi M
Formulation Research Institute, Otsuka Pharmaceutical Company, Tokushima, Japan.
Glycoconj J. 1992 Apr;9(2):99-108. doi: 10.1007/BF00731706.
The antigen structure of a mouse monoclonal antibody, GOM-2, established by immunization with KATO-III human gastric cancer cells, was examined. GOM-2 reactive glycolipids were prepared from KATO-III cells and treated with endoglycoceramidase. Structural studies of ten GOM-2 reactive oligosaccharides by a combination of glycosidase digestions, methylation, and affinity chromatography on an Ulex europeus agglutinin I (UEA-I) column revealed that nine of them had a Y-related B-active difucosylated determinant (B-Le(y)) and one had a B-active determinant. Affinity chromatography of the purified and modified oligosaccharides on an immobilized GOM-2 column demonstrated that GOM-2 has a novel binding specificity: it binds tightly to the biantennary structure carrying the B-Le(y) determinant at the termini or the branched structure carrying the B-Le(y) structure at two nonreducing termini.
研究了用KATO-III人胃癌细胞免疫制备的小鼠单克隆抗体GOM-2的抗原结构。从KATO-III细胞中制备出与GOM-2反应的糖脂，并用内切糖神经酰胺酶处理。通过糖苷酶消化、甲基化以及在欧洲荆豆凝集素I（UEA-I）柱上进行亲和色谱等方法，对10种与GOM-2反应的寡糖进行结构研究，结果表明其中9种具有Y相关的B活性二岩藻糖基化决定簇（B-Le(y)），1种具有B活性决定簇。在固定化GOM-2柱上对纯化和修饰后的寡糖进行亲和色谱分析表明，GOM-2具有一种新的结合特异性：它紧密结合于在末端带有B-Le(y)决定簇的双天线结构或在两个非还原末端带有B-Le(y)结构的分支结构。
