Ai Y, Tsai D S, Kao T H
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Plant Mol Biol. 1992 Jun;19(3):523-8. doi: 10.1007/BF00023404.
A defective S-allele, S(o), and a functional S-allele, Sx, have previously been found to be retained in an F1 hybrid of a self-compatible commercial cultivar of Petunia hybrida. Pistil proteins associated with these two alleles have also been identified. Their amino-terminal sequences have been found to share a high degree of similarity with those of S-proteins characterized from self-incompatible solanaceous species. Here we report the isolation and sequencing of cDNAs encoding S(o)- and Sx-proteins. Their deduced amino acid sequences contain all the consensus primary structural features of S-proteins from self-incompatible solanaceous species. Both proteins also have ribonuclease activity. The implications of these findings are discussed in relation to the presumed function of the S-protein in the self-incompatibility interaction.
先前已发现，矮牵牛一个自交亲和商业栽培品种的F1杂种中保留了一个缺陷型S等位基因S(o)和一个功能型S等位基因Sx。与这两个等位基因相关的雌蕊蛋白也已得到鉴定。已发现它们的氨基末端序列与自交不亲和茄科物种中鉴定出的S蛋白的氨基末端序列具有高度相似性。在此，我们报告了编码S(o)蛋白和Sx蛋白的cDNA的分离与测序。它们推导的氨基酸序列包含来自自交不亲和茄科物种的S蛋白的所有共有一级结构特征。这两种蛋白也都具有核糖核酸酶活性。结合S蛋白在自交不亲和相互作用中的假定功能，对这些发现的意义进行了讨论。
