• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of aqueous isopropanol to remove detergent from gels.

作者信息

Blank A, Sugiyama R H, Dekker C A

出版信息

Anal Biochem. 1982 Mar 1;120(2):267-75. doi: 10.1016/0003-2697(82)90347-5.

DOI:10.1016/0003-2697(82)90347-5
PMID:6178316
Abstract
摘要

相似文献

1
Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of aqueous isopropanol to remove detergent from gels.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后核酸水解酶的活性染色：使用异丙醇水溶液从凝胶中去除去污剂。
Anal Biochem. 1982 Mar 1;120(2):267-75. doi: 10.1016/0003-2697(82)90347-5.
2
A method for recovery of proteins from sodium dodecyl sulfate--polyacrylamide gels.
Anal Biochem. 1979 Apr 15;94(2):274-7. doi: 10.1016/0003-2697(79)90360-9.
3
Nuclease detection in SDS-polyacrylamide gel electrophoresis.SDS-聚丙烯酰胺凝胶电泳中的核酸酶检测
Anal Biochem. 1977 May 15;80(1):76-90. doi: 10.1016/0003-2697(77)90627-3.
4
The tritium labeling of small amounts of protein for analysis by electrophoresis on sodium dodecyl sulfate--polyacrylamide slab gels.通过在十二烷基硫酸钠 - 聚丙烯酰胺平板凝胶上进行电泳分析对少量蛋白质进行氚标记。
Anal Biochem. 1979 Jun;95(2):359-63. doi: 10.1016/0003-2697(79)90739-5.
5
Substrate-containing gel electrophoresis: sensitive detection of amylolytic, nucleolytic, and proteolytic enzymes.含底物凝胶电泳：淀粉酶、核酸酶和蛋白酶的灵敏检测
Anal Biochem. 1982 May 1;122(1):164-72. doi: 10.1016/0003-2697(82)90266-4.
6
Copper staining: a five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels.铜染色：一种用于十二烷基硫酸钠-聚丙烯酰胺凝胶的五分钟蛋白质染色法。
Anal Biochem. 1987 Nov 1;166(2):308-12. doi: 10.1016/0003-2697(87)90579-3.
7
Enhanced removal of detergent and recovery of enzymatic activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of casein in gel wash buffer.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后增强去污剂去除及酶活性恢复：在凝胶洗涤缓冲液中使用酪蛋白。
Anal Biochem. 1990 Aug 15;189(1):68-74. doi: 10.1016/0003-2697(90)90045-b.
8
Combined alcian blue and silver staining of subnanogram quantities of proteoglycans and glycosaminoglycans in sodium dodecyl sulfate-polyacrylamide gels.十二烷基硫酸钠-聚丙烯酰胺凝胶中亚纳克量级蛋白聚糖和糖胺聚糖的阿尔新蓝和银联合染色法
Anal Biochem. 1993 Feb 15;209(1):169-75. doi: 10.1006/abio.1993.1098.
9
Neutron-scattering probe of complexes of sodium dodecyl sulfate and serum albumin during polyacrylamide gel electrophoresis.聚丙烯酰胺凝胶电泳过程中十二烷基硫酸钠与血清白蛋白复合物的中子散射探测
Langmuir. 2009 Feb 3;25(3):1558-65. doi: 10.1021/la8039994.
10
Two-dimensional zymogram analysis of nucleases in Bacillus subtilis.
Anal Biochem. 1983 Sep;133(2):322-9. doi: 10.1016/0003-2697(83)90091-x.

引用本文的文献

1
A highly sensitive nanopore platform for measuring RNase A activity.一种用于测量 RNase A 活性的高灵敏度纳米孔平台。
Talanta. 2024 Aug 15;276:126276. doi: 10.1016/j.talanta.2024.126276. Epub 2024 May 22.
2
Detailed analysis of agro-industrial byproducts/wastes to enable efficient sorting for various agro-industrial applications.对农用工业副产品/废弃物进行详细分析，以便为各种农用工业应用进行高效分类。
Bioresour Bioprocess. 2024 May 4;11(1):45. doi: 10.1186/s40643-024-00763-7.
3
Hortensins, Type 1 Ribosome-Inactivating Proteins from Seeds of Red Mountain Spinach: Isolation, Characterization, and Their Effect on Glioblastoma Cells.
红山菠菜种子中的 1 型核糖体失活蛋白（Hortensins）：分离、鉴定及其对神经胶质瘤细胞的作用。
Toxins (Basel). 2024 Mar 4;16(3):135. doi: 10.3390/toxins16030135.
4
What Worth the Garlic Peel.大蒜皮的价值
Int J Mol Sci. 2022 Feb 15;23(4):2126. doi: 10.3390/ijms23042126.
5
Dead Pericarps of Dry Fruits Function as Long-Term Storage for Active Hydrolytic Enzymes and Other Substances That Affect Germination and Microbial Growth.干果的死果皮可作为活性水解酶和其他影响种子萌发及微生物生长的物质的长期储存库。
Plants (Basel). 2017 Dec 19;6(4):64. doi: 10.3390/plants6040064.
6
The dead seed coat functions as a long-term storage for active hydrolytic enzymes.死亡的种皮起到了活性水解酶长期储存库的作用。
PLoS One. 2017 Jul 11;12(7):e0181102. doi: 10.1371/journal.pone.0181102. eCollection 2017.
7
The dead, hardened floral bracts of dispersal units of wild wheat function as storage for active hydrolases and in enhancing seedling vigor.野生小麦传播单位中死亡、硬化的花苞片起到储存活性水解酶和增强幼苗活力的作用。
PLoS One. 2017 May 11;12(5):e0177537. doi: 10.1371/journal.pone.0177537. eCollection 2017.
8
S1-Type Endonuclease 2 in Dedifferentiating Arabidopsis Protoplasts: Translocation to the Nucleus in Senescing Protoplasts Is Associated with De-Glycosylation.拟南芥原生质体去分化过程中的S1型核酸内切酶2：衰老原生质体中向细胞核的转位与去糖基化有关。
PLoS One. 2017 Jan 9;12(1):e0170067. doi: 10.1371/journal.pone.0170067. eCollection 2017.
9
Multiple Patterns of Regulation and Overexpression of a Ribonuclease-Like Pathogenesis-Related Protein Gene, OsPR10a, Conferring Disease Resistance in Rice and Arabidopsis.一种类似核糖核酸酶的病程相关蛋白基因OsPR10a的多种调控和过表达模式赋予水稻和拟南芥抗病性
PLoS One. 2016 Jun 3;11(6):e0156414. doi: 10.1371/journal.pone.0156414. eCollection 2016.
10
Influence of Conformation of M. tuberculosis RNase P Protein Subunit on Its Function.结核分枝杆菌核糖核酸酶P蛋白亚基构象对其功能的影响
PLoS One. 2016 Apr 18;11(4):e0153798. doi: 10.1371/journal.pone.0153798. eCollection 2016.